For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    dopa-decarboxylaseddc-antibody-cl2962-ab211535.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurotransmitter Biogenic Amines Dopamine
Share by email

Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)

  • Datasheet
  • SDS
Submit a review Submit a question References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)
  • Immunocytochemistry/ Immunofluorescence - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)

Key features and details

  • Mouse monoclonal [CL2962] to DOPA Decarboxylase/DDC
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG1

You may also be interested in

Primary
Product image
Anti-TPH2 antibody (ab121013)
Biochemical
Product image
2',3'-Dideoxycytidine (ddC), nucleoside reverse transcriptase inhibitor (NRTI) (ab142240)
Primary
Product image
Anti-Dopamine Transporter antibody [EPR19695] (ab184451)

View more associated products

Overview

  • Product name

    Anti-DOPA Decarboxylase/DDC antibody [CL2962]
    See all DOPA Decarboxylase/DDC primary antibodies
  • Description

    Mouse monoclonal [CL2962] to DOPA Decarboxylase/DDC
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Cow, Pig, Drosophila melanogasterDoes not react with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human DOPA Decarboxylase/DDC aa 100-250.
    Database link: P20711

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Epitope

    Binds to an epitope located within the peptide sequence MDWLGKMLEL (aa 119-128) as determined by overlapping synthetic peptides.
  • Positive control

    • IHC: Rat substantia nigra and human substantia nigra tissues. WB: Human kidney tissue lysate ICC/IF: Rat substantia nigra tissue
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    CL2962
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Dopamine
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human DOPA Decarboxylase/DDC protein (ab85332)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab211535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/1000. Predicted molecular weight: 53 kDa.
IHC-P
1/1000 - 1/2500.
ICC/IF
Use at an assay dependent concentration.
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 53 kDa.
IHC-P
1/1000 - 1/2500.
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.
  • Pathway

    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 2/2.
  • Involvement in disease

    Defects in DDC are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD) [MIM:608643]. AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. It causes developmental and psychomotor delay, poor feeding, lethargy, ptosis, intermittent hypothermia, gastrointestinal disturbances. The onset is early in infancy and inheritance is autosomal recessive.
  • Sequence similarities

    Belongs to the group II decarboxylase family.
  • Target information above from: UniProt accession P20711 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 280762 Cow
    • Entrez Gene: 35190 Drosophila melanogaster
    • Entrez Gene: 1644 Human
    • Entrez Gene: 24311 Rat
    • Omim: 107930 Human
    • SwissProt: P27718 Cow
    • SwissProt: P05031 Drosophila melanogaster
    • SwissProt: P20711 Human
    • SwissProt: P80041 Pig
    • SwissProt: P14173 Rat
    • Unigene: 12979 Drosophila melanogaster
    • Unigene: 359698 Human
    • Unigene: 11064 Rat
    see all
  • Alternative names

    • AADC antibody
    • Aromatic L Amino Acid Decarboxylase antibody
    • Aromatic-L-amino-acid decarboxylase antibody
    • DDC antibody
    • DDC_HUMAN antibody
    • DOPA decarboxylase (aromatic L-amino acid decarboxylase) antibody
    • DOPA decarboxylase antibody
    see all

Images

  • Western blot - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)
    Western blot - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)
    Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535) at 1/500 dilution + Human kidney tissue lysate

    Predicted band size: 53 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)

    Immunohistochemical analysis of human kidney tissue staining DDC with ab211535 at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)
    Immunocytochemistry/ Immunofluorescence - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)

    Immunocytochemistry/Immunofluorescent analysis of rat substantia nigra cells staining DOPA Decarboxylase with ab211535.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)

    Immunohistochemical analysis of paraffin-embedded human substantia nigra tissue labeling DOPA Decarboxylase/DDC with ab211535 at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)

    Immunohistochemical analysis of human fallopian tube tissue staining DDC with ab211535 at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)

    Immunohistochemical analysis of paraffin-embedded rat substantia nigra tissue labeling DOPA Decarboxylase/DDC with ab211535 at 1/1000 dilution.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab211535? Please let us know so that we can cite the reference in this datasheet.

ab211535 has been referenced in 2 publications.

  • Britto-Júnior J  et al. Endothelium-derived dopamine modulates EFS-induced contractions of human umbilical vessels. Pharmacol Res Perspect 8:e00612 (2020). PubMed: 32567793
  • Kim H  et al. Modeling G2019S-LRRK2 Sporadic Parkinson's Disease in 3D Midbrain Organoids. Stem Cell Reports 12:518-531 (2019). PubMed: 30799274

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab211535.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.