Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)
Key features and details
- Mouse monoclonal [CL2962] to DOPA Decarboxylase/DDC
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-DOPA Decarboxylase/DDC antibody [CL2962]
See all DOPA Decarboxylase/DDC primary antibodies -
Description
Mouse monoclonal [CL2962] to DOPA Decarboxylase/DDC -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Cow, Pig, Drosophila melanogasterDoes not react with: Mouse
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Immunogen
Recombinant fragment corresponding to Human DOPA Decarboxylase/DDC aa 100-250.
Database link: P20711 -
Epitope
Binds to an epitope located within the peptide sequence MDWLGKMLEL (aa 119-128) as determined by overlapping synthetic peptides. -
Positive control
- IHC: Rat substantia nigra and human substantia nigra tissues. WB: Human kidney tissue lysate ICC/IF: Rat substantia nigra tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL2962 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 53 kDa.
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IHC-P |
1/1000 - 1/2500.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 53 kDa. |
IHC-P
1/1000 - 1/2500. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. -
Pathway
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 2/2. -
Involvement in disease
Defects in DDC are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD) [MIM:608643]. AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. It causes developmental and psychomotor delay, poor feeding, lethargy, ptosis, intermittent hypothermia, gastrointestinal disturbances. The onset is early in infancy and inheritance is autosomal recessive. -
Sequence similarities
Belongs to the group II decarboxylase family. - Information by UniProt
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Database links
- Entrez Gene: 280762 Cow
- Entrez Gene: 35190 Drosophila melanogaster
- Entrez Gene: 1644 Human
- Entrez Gene: 24311 Rat
- Omim: 107930 Human
- SwissProt: P27718 Cow
- SwissProt: P05031 Drosophila melanogaster
- SwissProt: P20711 Human
see all -
Alternative names
- AADC antibody
- Aromatic L Amino Acid Decarboxylase antibody
- Aromatic-L-amino-acid decarboxylase antibody
see all
Images
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Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535) at 1/500 dilution + Human kidney tissue lysate
Predicted band size: 53 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)
Immunohistochemical analysis of human kidney tissue staining DDC with ab211535 at 1/1000 dilution.
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Immunocytochemistry/Immunofluorescent analysis of rat substantia nigra cells staining DOPA Decarboxylase with ab211535.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)
Immunohistochemical analysis of paraffin-embedded human substantia nigra tissue labeling DOPA Decarboxylase/DDC with ab211535 at 1/1000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)
Immunohistochemical analysis of human fallopian tube tissue staining DDC with ab211535 at 1/1000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOPA Decarboxylase/DDC antibody [CL2962] (ab211535)
Immunohistochemical analysis of paraffin-embedded rat substantia nigra tissue labeling DOPA Decarboxylase/DDC with ab211535 at 1/1000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab211535 has been referenced in 2 publications.
- Britto-Júnior J et al. Endothelium-derived dopamine modulates EFS-induced contractions of human umbilical vessels. Pharmacol Res Perspect 8:e00612 (2020). PubMed: 32567793
- Kim H et al. Modeling G2019S-LRRK2 Sporadic Parkinson's Disease in 3D Midbrain Organoids. Stem Cell Reports 12:518-531 (2019). PubMed: 30799274