Anti-DOPA Decarboxylase/DDC antibody - N-terminal (ab189101)
Key features and details
- Rabbit polyclonal to DOPA Decarboxylase/DDC - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Cow, Human
- Isotype: IgG
Overview
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Product name
Anti-DOPA Decarboxylase/DDC antibody - N-terminal
See all DOPA Decarboxylase/DDC primary antibodies -
Description
Rabbit polyclonal to DOPA Decarboxylase/DDC - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Cow, Human
Predicted to work with: Rat, Sheep, Guinea pig, Dog -
Immunogen
Synthetic peptide corresponding to Human DOPA Decarboxylase/DDC (N terminal) conjugated to keyhole limpet haemocyanin. (NP_000781)
Database link: P20711 -
Positive control
- Human caudate and bovine adrenal medulla lysates; Human kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituents: 0.87% Sodium chloride, 0.24% HEPES, 50% Glycerol (glycerin, glycerine), 0.01% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/1000. Predicted molecular weight: 53 kDa.
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Notes |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/1000. Predicted molecular weight: 53 kDa. |
Target
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Function
Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. -
Pathway
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 2/2. -
Involvement in disease
Defects in DDC are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD) [MIM:608643]. AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. It causes developmental and psychomotor delay, poor feeding, lethargy, ptosis, intermittent hypothermia, gastrointestinal disturbances. The onset is early in infancy and inheritance is autosomal recessive. -
Sequence similarities
Belongs to the group II decarboxylase family. - Information by UniProt
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Database links
- Entrez Gene: 280762 Cow
- Entrez Gene: 1644 Human
- Entrez Gene: 24311 Rat
- Entrez Gene: 101106765 Sheep
- Omim: 107930 Human
- SwissProt: P27718 Cow
- SwissProt: P20711 Human
- SwissProt: P14173 Rat
see all -
Alternative names
- AADC antibody
- Aromatic L Amino Acid Decarboxylase antibody
- Aromatic-L-amino-acid decarboxylase antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling DOPA Decarboxylase/DDC with ab189101 at 1/100.
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Anti-DOPA Decarboxylase/DDC antibody - N-terminal (ab189101) at 1/1000 dilution + Human caudate lysate at 20 µg
Predicted band size: 53 kDa -
Anti-DOPA Decarboxylase/DDC antibody - N-terminal (ab189101) at 1/1000 dilution + Bovine adrenal medulla lysate at 5 µg
Predicted band size: 53 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab189101 has been referenced in 1 publication.
- Khoury S et al. A functional substitution in the L-aromatic amino acid decarboxylase enzyme worsens somatic symptoms via a serotonergic pathway. Ann Neurol 86:168-180 (2019). PubMed: 31177555