• Product name
  • Description
    Rabbit polyclonal to Dopamine
  • Host species
  • Specificity
    Using a conjugate Dopamine-Glutaraldehyde-bovine serum albumin, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
    G-BSA-conjugateCross-reactivity ratio (a)
    L-DOPA, Octopamine1/400
    Tyramine, Adrenalin, unconjugated Dopamine 1/>5,000
    (a): Dopamine-G-BSA concentration/unconjugated or conjugated catecholamine concentration at half displacement (b): Non-reduced conjugate
  • Tested applications
    Suitable for: ICC, ICC/IF, ELISAmore details
  • Immunogen

    Chemical/ Small Molecule by a Glutaraldehyde linker.

  • General notes

    The antiserum was tested using the free floating PAP technique on rat dopaminergic areas. The anti-conjugated Dopamine antibodies gave good staining between 1/2000-1/5000 dilution in these areas.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Primary antibody notes
    The antiserum was tested using the free floating PAP technique on rat dopaminergic areas. The anti-conjugated Dopamine antibodies gave good staining between 1/2000-1/5000 dilution in these areas.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab6427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000. Glutharaldehyde perfusion fixation (see recommended protocol).
ELISA 1/5000.


  • Relevance
    Dopamine (C6H3OH)2-CH2CH2NH2)is a catecholamine neurotransmitter in the brain. Its chemical name is 4-(2-aminoethyl)benzene-1,2-diol. Dopamine is a hormone released by the hypothalamus. Its main function is to inhibit the release of prolactin from the anterior lobe of the pituitary. It can be used as a sympathomimetic drug, producing effects such as increased heart rate and blood pressure.
  • Cellular localization
  • Alternative names
    • 3 4 dihydroxyphenethylamine antibody
    • 3 hydroxytyramine antibody
    • DA antibody
    • Dopamine antibody
    • Oxytyramine antibody
    see all


  • ab6427 at 1/5000 staining human stem cells (differentiated to neurons) by ICC. The cells were paraformaldehyde fixed and blocked with BSA before incubation with the antibody for 2 hours. A PE conjugated goatanti-rabbit antibody was used as the secondary.

    See Abreview

  • ab6427 staining dopamine in PC12 cells treated with ghrelin (rat) (ab120231), by ICC/IF. Decrease in dopamine expression correlates with increased concentration of ghrelin, as described in literature.
    The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120231 (ghrelin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6427 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.


This product has been referenced in:
  • Banks DA  et al. MK-STYX Alters the Morphology of Primary Neurons, and Outgrowths in MK-STYX Overexpressing PC-12 Cells Develop a Neuronal Phenotype. Front Mol Biosci 4:76 (2017). ICC/IF ; Rat . Read more (PubMed: 29250526) »
  • Wu SF  et al. Dopamine modulates hemocyte phagocytosis via a D1-like receptor in the rice stem borer, Chilo suppressalis. Sci Rep 5:12247 (2015). Read more (PubMed: 26179416) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

ab6427 is not guaranteed for use in immunohistochemistry. However, ab1001 (https://www.abcam.com/ab1001) is guaranteed to work in immunohistochemistry and gave good results when fixed with formalin/PFA in paraff...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Human Cell (stem cells differentiated to neurons)
stem cells differentiated to neurons
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 2

Abcam user community

Verified customer

Submitted May 29 2007

We have additional information about capturing the DA as follows: After discussion with our research team, we think that the better way is to bind DA on your tissue via glutaraldehyde (0.1 to 1%). - Double bounds are reduced by adding of sodium bor...

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Thank you for your enquiry. For Immunohistological studies with dopamine antibodies, fixation with glutaraldehyde is required. Please find below a protocol. Perfusion protocol for adult male Sprague-Dawley (weight around 0.5 kg) 1- The animals ca...

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The pump that was used is a peristaltic pump with a flow of 150-300ml/minute. GILSON supply this pump type.

This antibody is known to work with 3-5% glutaraldehyde perfusion. Protocol is as follows - Perfusion : perfuse intracardially through the aorta using a pump with the following solutions : solution A (30ml) : 200-300ml/min solution B (500ml) : 200-...

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