Key features and details
- Rabbit polyclonal to Dopamine
- Suitable for: IHC-P, IHC-Fr, ICC
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-Dopamine antibody
See all Dopamine primary antibodies
DescriptionRabbit polyclonal to Dopamine
Specificityab8888 targets conjugated dopamine, it does not recognize free dopamine.
Tested applicationsSuitable for: IHC-P, IHC-Fr, ICCmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to Dopamine conjugated to Bovine Serum Albumin (BSA).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: ddH20, 50% Glycerol
Concentration information loading...
Our Abpromise guarantee covers the use of ab8888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/5000. Fixation of tissue for use with this antibody should be done with gluteraldehyde. The use of paraformaldehyde in conjunction with gluteraldehyde may improve staining results.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
RelevanceDopamine (C6H3OH)2-CH2CH2NH2)is a catecholamine neurotransmitter in the brain. Its chemical name is 4-(2-aminoethyl)benzene-1,2-diol. Dopamine is a hormone released by the hypothalamus. Its main function is to inhibit the release of prolactin from the anterior lobe of the pituitary. It can be used as a sympathomimetic drug, producing effects such as increased heart rate and blood pressure.
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ab8888 at 1/500 staining human adrenal tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum before incubation with the antibody for 30 minutes at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ab8888 has been referenced in 9 publications.
- Auletta A et al. Tyrosine hydroxylase immunolabeling reveals the distribution of catecholaminergic neurons in the central nervous systems of the spiders Hogna lenta (Araneae: Lycosidae) and Phidippus regius (Araneae: Salticidae). J Comp Neurol N/A:N/A (2019). PubMed: 31343075
- Lu TM et al. Gene expression profiles of dicyemid life-cycle stages may explain how dispersing larvae locate new hosts. Zoological Lett 5:32 (2019). PubMed: 31754455
- Crossley M et al. A central control circuit for encoding perceived food value. Sci Adv 4:eaau9180 (2018). PubMed: 30474061
- Wolff GH et al. An insect-like mushroom body in a crustacean brain. Elife 6:N/A (2017). PubMed: 28949916
- Zieger E et al. The origin of dopaminergic systems in chordate brains: insights from amphioxus. Int J Dev Biol 61:749-761 (2017). PubMed: 29319121
- Xenias HS et al. Are striatal tyrosine hydroxylase interneurons dopaminergic? J Neurosci 35:6584-99 (2015). IHC ; Mouse . PubMed: 25904808
- Simo L et al. Evidence for d1 dopamine receptor activation by a paracrine signal of dopamine in tick salivary glands. PLoS One 6:e16158 (2011). IHC-Fr . PubMed: 21297964
- Prandovszky E et al. The neurotropic parasite toxoplasma gondii increases dopamine metabolism. PLoS One 6:e23866 (2011). IHC-P . PubMed: 21957440
- Outeiro TF et al. Dopamine-induced conformational changes in alpha-synuclein. PLoS One 4:e6906 (2009). ICC ; Human . PubMed: 19730729