Product nameAnti-Dopamine antibody
See all Dopamine primary antibodies
DescriptionRabbit polyclonal to Dopamine
Specificityab8888 targets conjugated dopamine, it does not recognize free dopamine.
Tested applicationsSuitable for: IHC-P, IHC-Fr, ICCmore details
Chemical/ Small Molecule corresponding to Dopamine conjugated to Bovine Serum Albumin (BSA).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: ddH20, 50% Glycerol
Our Abpromise guarantee covers the use of ab8888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/5000. Fixation of tissue for use with this antibody should be done with gluteraldehyde. The use of paraformaldehyde in conjunction with gluteraldehyde may improve staining results.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
RelevanceDopamine (C6H3OH)2-CH2CH2NH2)is a catecholamine neurotransmitter in the brain. Its chemical name is 4-(2-aminoethyl)benzene-1,2-diol. Dopamine is a hormone released by the hypothalamus. Its main function is to inhibit the release of prolactin from the anterior lobe of the pituitary. It can be used as a sympathomimetic drug, producing effects such as increased heart rate and blood pressure.
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ab8888 at 1/500 staining human adrenal tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum before incubation with the antibody for 30 minutes at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
This product has been referenced in:
- Wolff GH et al. An insect-like mushroom body in a crustacean brain. Elife 6:N/A (2017). Read more (PubMed: 28949916) »
- Xenias HS et al. Are striatal tyrosine hydroxylase interneurons dopaminergic? J Neurosci 35:6584-99 (2015). IHC ; Mouse . Read more (PubMed: 25904808) »