Anti-Dopamine beta Hydroxylase antibody (ab19353)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-Dopamine beta Hydroxylase antibody
See all Dopamine beta Hydroxylase primary antibodies -
DescriptionSheep polyclonal to Dopamine beta Hydroxylase
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Host speciesSheep
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Tested applicationsSuitable for: ICC, WB, ICC/IF, IHC-P, IHC-Frmore details
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Species reactivityReacts with: Mouse, Rat, Cow, Human
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Immunogen
Synthetic peptide corresponding to Rat Dopamine beta Hydroxylase aa 43-62.
Sequence:SEPPESPFPYHIPLDPEGTL
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General notesDetects human, bovine, rat and mouse DBH. It is not effective for immunohistochemistry in human brain
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.05% Sodium azide
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PurityWhole antiserum
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ClonalityPolyclonal
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Research areas
Applications
Our Abpromise guarantee covers the use of ab19353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC | 1/2500. | |
WB | 1/5000. Detects a band of approximately 72 kDa. | |
ICC/IF | 1/5000. | |
IHC-P | 1/2000. | |
IHC-Fr | Use at an assay dependent concentration. |
Target
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FunctionConversion of dopamine to noradrenaline.
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PathwayCatecholamine biosynthesis; (R)-noradrenaline biosynthesis; (R)-noradrenaline from dopamine: step 1/1.
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Involvement in diseaseDefects in DBH are the cause of dopamine beta-hydroxylase deficiency (DBH deficiency) [MIM:223360]; also known as norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction.
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Sequence similaritiesBelongs to the copper type II ascorbate-dependent monooxygenase family.
Contains 1 DOMON domain. -
Cellular localizationCytoplasmic vesicle > secretory vesicle lumen. Cytoplasmic vesicle > secretory vesicle > chromaffin granule lumen and Cytoplasmic vesicle > secretory vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane.
- Information by UniProt
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Database links
- Entrez Gene: 280758 Cow
- Entrez Gene: 1621 Human
- Entrez Gene: 13166 Mouse
- Entrez Gene: 25699 Rat
- Omim: 609312 Human
- SwissProt: P15101 Cow
- SwissProt: P09172 Human
- SwissProt: Q64237 Mouse
see all -
Alternative names
- dbh antibody
- DBM antibody
- Dopamine beta hydroxylase antibody
see all
Images
Protocols
References
This product has been referenced in:
- Mahata SK et al. Effect of heart failure on catecholamine granule morphology and storage in chromaffin cells. J Endocrinol 230:309-23 (2016). Read more (PubMed: 27402067) »
- Talavera-Adame D et al. Enhancement of embryonic stem cell differentiation promoted by avian chorioallantoic membranes. Tissue Eng Part A 15:3193-200 (2009). Read more (PubMed: 19364272) »