Anti-Dopamine beta Hydroxylase antibody (ab19353)

Abreviews (2)Q&A (4)Specific References (2)

Overview

  • Product name
    Anti-Dopamine beta Hydroxylase antibody
    See all Dopamine beta Hydroxylase primary antibodies
  • Description
    Sheep polyclonal to Dopamine beta Hydroxylase
  • Host species
    Sheep
  • Tested applications
    Suitable for: ICC, WB, ICC/IF, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Rat Dopamine beta Hydroxylase aa 43-62.
    Sequence:

    SEPPESPFPYHIPLDPEGTL

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes
    Detects human, bovine, rat and mouse DBH. It is not effective for immunohistochemistry in human brain

Properties

Applications

Our Abpromise guarantee covers the use of ab19353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/2500.
WB 1/5000. Detects a band of approximately 72 kDa.
ICC/IF 1/5000.
IHC-P 1/2000.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Conversion of dopamine to noradrenaline.
  • Pathway
    Catecholamine biosynthesis; (R)-noradrenaline biosynthesis; (R)-noradrenaline from dopamine: step 1/1.
  • Involvement in disease
    Defects in DBH are the cause of dopamine beta-hydroxylase deficiency (DBH deficiency) [MIM:223360]; also known as norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction.
  • Sequence similarities
    Belongs to the copper type II ascorbate-dependent monooxygenase family.
    Contains 1 DOMON domain.
  • Cellular localization
    Cytoplasmic vesicle > secretory vesicle lumen. Cytoplasmic vesicle > secretory vesicle > chromaffin granule lumen and Cytoplasmic vesicle > secretory vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • dbh antibody
    • DBM antibody
    • Dopamine beta hydroxylase antibody
    • Dopamine beta monooxygenase antibody
    • Dopamine beta-hydroxylase (dopamine beta-monooxygenase) antibody
    • Dopamine beta-monooxygenase antibody
    • DOPO_HUMAN antibody
    • OTTHUMP00000022501 antibody
    • Soluble dopamine beta-hydroxylase antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Dopamine beta Hydroxylase antibody (ab19353)
    Immunocytochemistry/ Immunofluorescence - Anti-Dopamine beta Hydroxylase antibody (ab19353)

    Immunofluorescent staining of DBH in rat neuron cells using ab19353.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody (ab19353)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody (ab19353)

    Immunofluorescent stain of DBH in rat brain tissue using ab19353.

References

This product has been referenced in:
  • Mahata SK  et al. Effect of heart failure on catecholamine granule morphology and storage in chromaffin cells. J Endocrinol 230:309-23 (2016). Read more (PubMed: 27402067) »
  • Talavera-Adame D  et al. Enhancement of embryonic stem cell differentiation promoted by avian chorioallantoic membranes. Tissue Eng Part A 15:3193-200 (2009). Read more (PubMed: 19364272) »

See all 2 Publications for this product

Publishing research using ab19353? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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6 reviews or Q&A

10
Votes: Highest First

Immunohistochemistry free floating abreview for Anti-Dopamine beta Hydroxylase antibody

 Inconclusive
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Mouse brain and spinal cord)
Specification
Mouse brain and spinal cord
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Verified customer

Submitted Feb 22 2017

Immunohistochemistry - Free Floating abreview for Anti-Dopamine beta Hydroxylase antibody

 Average
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain: Coronal sections of striatum and cortex)
Specification
Brain: Coronal sections of striatum and cortex
Read More
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Abcam user community

Verified customer

Submitted Jan 28 2016

trying ab19353 in IHC-Fr
mouse brain sections, PFA fix o/n

DISCOUNT CODE: xxx
Expiration date: xxx
Value: xxx

I am very pleased to hear you would like to accept our offer and test ab19353 in IHC-Fr. This code will give you xxx off your next order before the expiration date. To redeem this off...

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voici le questionnaire rempli avec le western blot. 

 Cordialement

Merci beaucoup de votre réponse et de vous avoir pris le temps de remplir le questionnaire.

Effectivement le point qui saute à l'œilen regardant leprotocole, est la méthode d'extraction de la protéine et pr&e...

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phone call: no band at expected size on mouse brain lysate

Je suis désolée d'apprendre que ab19353 ne vous donne pas de bons résultats en Western blot.

Veuillez trouver ci-jointlequestionnaire discuté au téléphone qui nous permet de regrouper le plus d'inform...

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Thank you for the prompt reply.  The animal was perfusion-fixed with 4% PFA + 0.1% glutaraldehyde. The spinal cord was dissected and stored in PBS, and rinsed several times with the same buffer before  immersion in 30% sucrose. Antigen retrieval was no...

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Thank you for sending your protocol information. We haven't tested this antibody with perfusion-fixed or free floating tissue, but we generally recommend using an antigen retrieval method with formaldehyde-fixed tissue, as formaldehyde crosslinks p...

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