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Neuroscience Neurotransmitter Biogenic Amines Dopamine
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RecombinantRabMAb

Recombinant Anti-Dopamine beta Hydroxylase antibody - BSA and Azide free (Capture) (ab277245)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal to Dopamine beta Hydroxylase - BSA and Azide free (Capture)
  • Suitable for: Sandwich ELISA
  • Reacts with: Human

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Overview

  • Product name

    Anti-Dopamine beta Hydroxylase antibody - BSA and Azide free (Capture)
    See all Dopamine beta Hydroxylase primary antibodies
  • Description

    Rabbit monoclonal to Dopamine beta Hydroxylase - BSA and Azide free (Capture)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human
  • General notes

    ab277245 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Dopamine beta Hydroxylase. The recommended pair for sandwich ELISA is:
              Capture: ab277245, Human Dopamine beta Hydroxylase Capture Antibody (unconjugated)
              Detector: ab277246, Human Dopamine beta Hydroxylase Detector Antibody (unconjugated)
    The reference range value is 78.125 - 5000 pg/ml.

    The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Constituent: 100% PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Dopamine
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (Biotin) (ab207995)
    • Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
  • Conjugation kits

    • Alkaline phosphatase Conjugation Kit - Lightning-Link® (ab102850)
    • HRP Conjugation Kit - Lightning-Link® (ab102890)
    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • sELISA pair antibody

    • Anti-Dopamine beta Hydroxylase antibody - BSA and Azide free (Detector) (ab277246)

Applications

Our Abpromise guarantee covers the use of ab277245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal to Dopamine beta Hydroxylase - BSA and Azide free (Detector) (ab277246).

Target

  • Function

    Conversion of dopamine to noradrenaline.
  • Pathway

    Catecholamine biosynthesis; (R)-noradrenaline biosynthesis; (R)-noradrenaline from dopamine: step 1/1.
  • Involvement in disease

    Defects in DBH are the cause of dopamine beta-hydroxylase deficiency (DBH deficiency) [MIM:223360]; also known as norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction.
  • Sequence similarities

    Belongs to the copper type II ascorbate-dependent monooxygenase family.
    Contains 1 DOMON domain.
  • Cellular localization

    Cytoplasmic vesicle > secretory vesicle lumen. Cytoplasmic vesicle > secretory vesicle > chromaffin granule lumen and Cytoplasmic vesicle > secretory vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane.
  • Target information above from: UniProt accession P09172 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1621 Human
    • Omim: 609312 Human
    • SwissProt: P09172 Human
    • Unigene: 591890 Human
    • Alternative names

      • dbh antibody
      • DBM antibody
      • Dopamine beta hydroxylase antibody
      • Dopamine beta monooxygenase antibody
      • Dopamine beta-hydroxylase (dopamine beta-monooxygenase) antibody
      • Dopamine beta-monooxygenase antibody
      • DOPO_HUMAN antibody
      • OTTHUMP00000022501 antibody
      • Soluble dopamine beta-hydroxylase antibody
      see all

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab277245? Please let us know so that we can cite the reference in this datasheet.

    ab277245 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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