Recombinant Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487)](https://www.abcam.com/ps/products/209/ab209487/Images/ab209487-273369-anti-dopamine-beta-hydroxylase-antibody-epr20385-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20385] to Dopamine beta Hydroxylase
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Dopamine beta Hydroxylase antibody [EPR20385]
See all Dopamine beta Hydroxylase primary antibodies -
Description
Rabbit monoclonal [EPR20385] to Dopamine beta Hydroxylase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: SH-SY5Y whole cell lysate; Mouse and rat adrenal gland lysates. IHC-P: Human adrenal gland and adrenal pheochromocytoma tissues; Mouse and rat adrenal gland tissues. IP: SH-SY5Y whole cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20385 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab209487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 69 kDa).
|
|
| IHC-P | (4) |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Tris/EDTA Buffer, pH9 (ab93684) |
| IP |
1/40.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 69 kDa). |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Tris/EDTA Buffer, pH9 (ab93684) |
|
IP
1/40. |
Target
-
Function
Conversion of dopamine to noradrenaline. -
Pathway
Catecholamine biosynthesis; (R)-noradrenaline biosynthesis; (R)-noradrenaline from dopamine: step 1/1. -
Involvement in disease
Defects in DBH are the cause of dopamine beta-hydroxylase deficiency (DBH deficiency) [MIM:223360]; also known as norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction. -
Sequence similarities
Belongs to the copper type II ascorbate-dependent monooxygenase family.
Contains 1 DOMON domain. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle lumen. Cytoplasmic vesicle > secretory vesicle > chromaffin granule lumen and Cytoplasmic vesicle > secretory vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. - Information by UniProt
-
Database links
- Entrez Gene: 1621 Human
- Entrez Gene: 13166 Mouse
- Entrez Gene: 25699 Rat
- Omim: 609312 Human
- SwissProt: P09172 Human
- SwissProt: Q64237 Mouse
- SwissProt: Q05754 Rat
- Unigene: 591890 Human
see all -
Alternative names
- dbh antibody
- DBM antibody
- Dopamine beta hydroxylase antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487)
Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue labeling Dopamine beta Hydroxylase with ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Strong cytoplasmic staining on chromaffin cells of human adrenal gland medulla, and negative on adrenal cortex (PMID: 17535872, PMID: 17699566).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Western blot - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487)](https://www.abcam.com/ps/products/209/ab209487/Images/ab209487-273371-anti-dopamine-beta-hydroxylase-antibody-epr20385-western-blot.jpg)
Western blot - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487)All lanes : Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487) at 1/1000 dilution
Lane 1 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 69 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Negative controls: HeLa (PMID: 7688735; PMID: 9763470, PMID:15585414, PMID: 10037744), C6 (PMID: 9763470, PMID:7798950, PMID: 10037744), HepG2 (PMID:7798950, PMID:11943777, PMID:15585414).
-
![Immunoprecipitation - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487)](https://www.abcam.com/ps/products/209/ab209487/Images/ab209487-273365-anti-dopamine-beta-hydroxylase-antibody-epr20385-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487)Dopamine beta Hydroxylase was immunoprecipitated from 0.35 mg of SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate with ab209487 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab209487 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: SH-SY5Y whole cell lysate, 10 µg (Input).
Lane 2: ab209487 IP in SH-SY5Y whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209487 in SH-SY5Y whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487)](https://www.abcam.com/ps/products/209/ab209487/Images/ab209487-273368-anti-dopamine-beta-hydroxylase-antibody-epr20385-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487)Immunohistochemical analysis of paraffin-embedded human adrenal pheochromocytoma tissue labeling Dopamine beta Hydroxylase with ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on tumor cells of human adrenal pheochromocytoma is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Western blot - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487)](https://www.abcam.com/ps/products/209/ab209487/Images/ab209487-273370-anti-dopamine-beta-hydroxylase-antibody-epr20385-western-blot.jpg)
Western blot - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487)All lanes : Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487) at 1/1000 dilution
Lane 1 : Mouse adrenal gland lysate
Lane 2 : Rat adrenal gland lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 69 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 minutes; Lane 2: 1 second.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487)](https://www.abcam.com/ps/products/209/ab209487/Images/ab209487-273367-anti-dopamine-beta-hydroxylase-antibody-epr20385-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487)Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling Dopamine beta Hydroxylase with ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on chromaffin cells of mouse adrenal gland medulla, and negative on adrenal cortex (PMID: 17535872, PMID: 176995660).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487)](https://www.abcam.com/ps/products/209/ab209487/Images/ab209487-273366-anti-dopamine-beta-hydroxylase-antibody-epr20385-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487)Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Dopamine beta Hydroxylase with ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on chromaffin cells of rat adrenal gland medulla, and negative on adrenal cortex (PMID: 17535872, PMID: 176995660).
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487)](https://www.abcam.com/ps/products/209/ab209487/Images/ab209487-8-benefits-of-recombinant-antibodies.png)
Anti-Dopamine beta Hydroxylase antibody [EPR20385] (ab209487)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (7)
ab209487 has been referenced in 7 publications.
- Kimura N Dopamine ß-hydroxylase: An Essential and Optimal Immunohistochemical Marker for Pheochromocytoma and Sympathetic Paraganglioma. Endocr Pathol N/A:N/A (2021). PubMed: 33405069
- Sakayori N et al. Maternal dietary imbalance between omega-6 and omega-3 fatty acids triggers the offspring's overeating in mice. Commun Biol 3:473 (2020). PubMed: 32859990
- Maynard ME et al. Loss of PTEN-induced kinase 1 (Pink1) reduces hippocampal tyrosine hydroxylase and impairs learning and memory. Exp Neurol 323:113081 (2020). PubMed: 31655049
- Cleypool CGJ et al. Sympathetic nerve tissue in milky spots of the human greater omentum. J Anat 236:156-164 (2020). PubMed: 31498441
- Francisco A et al. Mitochondrial NAD(P)+ Transhydrogenase is Unevenly Distributed in Different Brain Regions, and its Loss Causes Depressive-like Behavior and Motor Dysfunction in Mice. Neuroscience 440:210-229 (2020). PubMed: 32497756
- Feng J et al. A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine. Neuron 102:745-761.e8 (2019). PubMed: 30922875
- Li Y et al. Investigation of Behavioral Dysfunctions Induced by Monoamine Depletions in a Mouse Model of Parkinson's Disease. Front Cell Neurosci 12:241 (2018). PubMed: 30135645