Recombinant
RabMAb

Recombinant Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free (ab223130)

Overview

  • Product name

    Anti-Dopamine beta Hydroxylase antibody [EPR20385] - BSA and Azide free
    See all Dopamine beta Hydroxylase primary antibodies
  • Description

    Rabbit monoclonal [EPR20385] to Dopamine beta Hydroxylase - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment aa 50-350. The exact sequence is proprietary.
    Database link: P09172

  • Positive control

    • WB: SH-SY5Y whole cell lysate; Mouse and rat adrenal gland lysates. IHC-P: Human adrenal gland and adrenal pheochromocytoma tissues; Mouse and rat adrenal gland tissues. IP: SH-SY5Y whole cell lysate.
  • General notes

    Ab223130 is the carrier-free version of ab209487. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab223130 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab223130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 69 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tris/EDTA Buffer, pH9 (ab93684)

IP Use at an assay dependent concentration.

Target

  • Function

    Conversion of dopamine to noradrenaline.
  • Pathway

    Catecholamine biosynthesis; (R)-noradrenaline biosynthesis; (R)-noradrenaline from dopamine: step 1/1.
  • Involvement in disease

    Defects in DBH are the cause of dopamine beta-hydroxylase deficiency (DBH deficiency) [MIM:223360]; also known as norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction.
  • Sequence similarities

    Belongs to the copper type II ascorbate-dependent monooxygenase family.
    Contains 1 DOMON domain.
  • Cellular localization

    Cytoplasmic vesicle > secretory vesicle lumen. Cytoplasmic vesicle > secretory vesicle > chromaffin granule lumen and Cytoplasmic vesicle > secretory vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • dbh antibody
    • DBM antibody
    • Dopamine beta hydroxylase antibody
    • Dopamine beta monooxygenase antibody
    • Dopamine beta-hydroxylase (dopamine beta-monooxygenase) antibody
    • Dopamine beta-monooxygenase antibody
    • DOPO_HUMAN antibody
    • OTTHUMP00000022501 antibody
    • Soluble dopamine beta-hydroxylase antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue labeling Dopamine beta Hydroxylase with ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Strong cytoplasmic staining on chromaffin cells of human adrenal gland medulla, and negative on adrenal cortex (PMID: 17535872, PMID: 17699566).

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209487).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human adrenal pheochromocytoma tissue labeling Dopamine beta Hydroxylase with ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on tumor cells of human adrenal pheochromocytoma is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209487).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling Dopamine beta Hydroxylase with ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on chromaffin cells of mouse adrenal gland medulla, and negative on adrenal cortex (PMID: 17535872, PMID: 176995660).

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209487).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Dopamine beta Hydroxylase with ab209487 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on chromaffin cells of rat adrenal gland medulla, and negative on adrenal cortex (PMID: 17535872, PMID: 176995660).

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209487).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Dopamine beta Hydroxylase was immunoprecipitated from 0.35 mg of SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate with ab209487 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab209487 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: SH-SY5Y whole cell lysate, 10 µg (Input).

    Lane 2: ab209487 IP in SH-SY5Y whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209487 in SH-SY5Y whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209487).

References

ab223130 has not yet been referenced specifically in any publications.

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