Overview

  • Product name

    Anti-Dopamine D2 Receptor antibody
    See all Dopamine D2 Receptor primary antibodies
  • Description

    Rabbit polyclonal to Dopamine D2 Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C-terminal amino acids 306-336 of Human Dopamine D2 Receptor (P14416, NP_057658.2, NP_000786.1).

  • Positive control

    • NIH 3T3 cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab130295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 51 kDa.

Target

  • Function

    This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
  • Involvement in disease

    Defects in DRD2 are associated with dystonia type 11 (DYT11) [MIM:159900]; also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • D(2) dopamine receptor antibody
    • D2 dopamine receptor antibody
    • D2DR antibody
    • D2R antibody
    • Dopamine D2 receptor antibody
    • Dopamine receptor D2 antibody
    • DRD 2 antibody
    • DRD2 antibody
    • DRD2_HUMAN antibody
    see all

Images

  • Anti-Dopamine D2 Receptor antibody (ab130295) at 1/2000 dilution + Human brain lysate at 20 µg with NFDM/TBST at 5 %

    Secondary
    Goat anti-rabbit IgG (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 51 kDa

  • Anti-Dopamine D2 Receptor antibody (ab130295) at 1/2000 dilution + Human cerebellum lysate at 20 µg with NFDM/TBST at 5 %

    Secondary
    Goat anti-rabbit IgG (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 51 kDa

  • Anti-Dopamine D2 Receptor antibody (ab130295) at 1/2000 dilution + K562 whole cell lysate at 20 µg with NFDM/TBST at 5 %

    Secondary
    Goat anti-rabbit IgG (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 51 kDa

  • Anti-Dopamine D2 Receptor antibody (ab130295) at 1/2000 dilution + HT-1080 whole cell lysate at 20 µg with NFDM/TBST at 5 %

    Secondary
    Goat anti-rabbit IgG (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 51 kDa

  • Anti-Dopamine D2 Receptor antibody (ab130295) at 1/100 dilution + NIH 3T3 cell line lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 51 kDa

References

ab130295 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse organ)
Gel Running Conditions
Reduced Denaturing (50 degree for 15 min in loading buffer with DTT)
Loading amount
100 µg
Specification
Mouse organ
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Miss. Yao Liu

Verified customer

Submitted Mar 15 2019

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Heat-Inactivated Normal Donkey Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - Tween-20
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 18 2014

Answer

The datasheet for ab130295 shows a nice clean blot but just one sample, a cell lysate, on a blot stained with antibody diluted 1/100. The datasheet for ab32349 shows a messier blot stained at 1/1000 but the samples, a variety of tissue homogenates, are more like your sample, in particular lane 1. Tissue samples typically give dirtier blots.

Depsite the cross-reaction at 37 kDa (we do not know what this band represents) and weaker cross-reaction at other molecular weights, I recommend the rabbit polyclonal ab32349. However, both antibodies are covered by our guarantee for mouse WB, so if either fails, we can send a replacement, assuming we can find no way to improve you results with protocol modifications.

Read More

Answer

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions

- What is the problem with these products. Please fill in the attached relevant questionnaire.
- There are 8 antibodies in this order but the problem is submitted for 5. Is it true 3 others have worked or yet to be tried by customers.
- At what temperature customer stored the products?

Thank you very much for your cooperation. I will look forward to hearing from you soon.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up