Anti-Dopamine D2 Receptor antibody (ab30743)
- Datasheet
- References
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Overview
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Product nameAnti-Dopamine D2 Receptor antibody
See all Dopamine D2 Receptor primary antibodies -
DescriptionGoat polyclonal to Dopamine D2 Receptor
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Host speciesGoat
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Dog
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Immunogen
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Positive control
- This antibody gave a positive signal in the following Human adult normal tissue lysates: Brain, Liver, Heart and Skeletal Muscle This antibody gave a negative signal in the following Human adult normal tissue lysates: Testis, Kidney, Colon and Small Intestine
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: 1% BSA
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us. -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab30743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51, 48 kDa). | |
| ICC/IF | Use a concentration of 1 µg/ml. |
Target
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FunctionThis is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
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Involvement in diseaseDefects in DRD2 are associated with dystonia type 11 (DYT11) [MIM:159900]; also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable.
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Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
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Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 1813 Human
- Entrez Gene: 13489 Mouse
- Omim: 126450 Human
- SwissProt: P14416 Human
- SwissProt: P61168 Mouse
- Unigene: 73893 Human
- Unigene: 41970 Mouse
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Alternative names
- D(2) dopamine receptor antibody
- D2 dopamine receptor antibody
- D2DR antibody
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Images
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Western blot - Anti-Dopamine D2 Receptor antibody (ab30743)All lanes : Anti-Dopamine D2 Receptor antibody (ab30743) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lane 3 : Human liver tissue lysate - total protein (ab29889)
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 6 : Human kidney tissue lysate - total protein (ab30203)
Lane 7 : Human colon tissue lysate - total protein (ab30051)
Lane 8 : Human small intestine tissue lysate - total protein (ab29276)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit polyclonal to Goat IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 51, 48 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Dopamine D2 Receptor antibody (ab30743)ICC/IF image of ab30743 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30743, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96931 Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References
ab30743 has not yet been referenced specifically in any publications.
