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    dopamine-d2-receptor-antibody-ab30743.pdf

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Neuroscience Neurotransmitter Biogenic Amines Dopamine
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Anti-Dopamine D2 Receptor antibody (ab30743)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (2)

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Immunocytochemistry/ Immunofluorescence - Anti-Dopamine D2 Receptor antibody (ab30743)
  • Western blot - Anti-Dopamine D2 Receptor antibody (ab30743)
  • Immunocytochemistry/ Immunofluorescence - Anti-Dopamine D2 Receptor antibody (ab30743)

Key features and details

  • Goat polyclonal to Dopamine D2 Receptor
  • Suitable for: WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Dopamine D2 Receptor antibody
    See all Dopamine D2 Receptor primary antibodies
  • Description

    Goat polyclonal to Dopamine D2 Receptor
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Dog
  • Immunogen

    Synthetic peptide corresponding to Human Dopamine D2 Receptor aa 250-350 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab32347, ab32348)

  • Positive control

    • This antibody gave a positive signal in the following Human adult normal tissue lysates: Brain, Liver, Heart and Skeletal Muscle This antibody gave a negative signal in the following Human adult normal tissue lysates: Testis, Kidney, Colon and Small Intestine ICC/IF: B35 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: 1% BSA

    This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Dopamine
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Dopamine Receptors
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Dendrite marker

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Immunizing Peptide (Blocking)

    • Human Dopamine D2 Receptor peptide (ab32347)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human Dopamine D2 Receptor protein (ab112281)

Applications

Our Abpromise guarantee covers the use of ab30743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51, 48 kDa).Can be blocked with Human Dopamine D2 Receptor peptide (ab32347).
ICC/IF Use a concentration of 5 - 10 µg/ml.

Target

  • Function

    This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
  • Involvement in disease

    Defects in DRD2 are associated with dystonia type 11 (DYT11) [MIM:159900]; also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P14416 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1813 Human
    • Entrez Gene: 13489 Mouse
    • Entrez Gene: 24318 Rat
    • Omim: 126450 Human
    • SwissProt: P14416 Human
    • SwissProt: P61168 Mouse
    • SwissProt: P61169 Rat
    • Unigene: 73893 Human
    • Unigene: 41970 Mouse
    • Unigene: 87299 Rat
    see all
  • Alternative names

    • D(2) dopamine receptor antibody
    • D2 dopamine receptor antibody
    • D2DR antibody
    • D2R antibody
    • Dopamine D2 receptor antibody
    • Dopamine receptor D2 antibody
    • DRD 2 antibody
    • DRD2 antibody
    • DRD2_HUMAN antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Dopamine D2 Receptor antibody (ab30743)
    Immunocytochemistry/ Immunofluorescence - Anti-Dopamine D2 Receptor antibody (ab30743)

    ab30743 staining Dopamine D2 Receptor in B35 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab30743 at 5µg concentration and ab195884 (Rat monoclonal to Tubulin - Alexa Fluor® 647) at 1/100 dilution (shown in red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a donkey secondary antibody to goat IgG (Alexa Fluor® 488) (ab150133) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Anti-Dopamine D2 Receptor antibody (ab30743)
    Western blot - Anti-Dopamine D2 Receptor antibody (ab30743)
    All lanes : Anti-Dopamine D2 Receptor antibody (ab30743) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : Human heart tissue lysate - total protein (ab29431)
    Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 6 : Human kidney tissue lysate - total protein (ab30203)
    Lane 7 : Human colon tissue lysate - total protein (ab30051)
    Lane 8 : Human small intestine tissue lysate - total protein (ab29276)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Rabbit polyclonal to Goat IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 51, 48 kDa
    Observed band size: 51 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Dopamine D2 Receptor antibody (ab30743)
    Immunocytochemistry/ Immunofluorescence - Anti-Dopamine D2 Receptor antibody (ab30743)
    ICC/IF image of ab30743 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30743, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96931 Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab30743? Please let us know so that we can cite the reference in this datasheet.

    ab30743 has been referenced in 2 publications.

    • Yokoi R  et al. Impact of Sleep-Wake-Associated Neuromodulators and Repetitive Low-Frequency Stimulation on Human iPSC-Derived Neurons. Front Neurosci 13:554 (2019). PubMed: 31191238
    • Chabrat A  et al. Pharmacological Transdifferentiation of Human Nasal Olfactory Stem Cells into Dopaminergic Neurons. Stem Cells Int 2019:2945435 (2019). PubMed: 31236114

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-Dopamine D2 Receptor antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (SH-SY5Y)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    SH-SY5Y
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Miss. Yao Liu

    Verified customer

    Submitted Mar 12 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Dopamine D2 Receptor antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (SH-SY5Y)
    Permeabilization
    No
    Specification
    SH-SY5Y
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10µg/mL · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Miss. Yao Liu

    Verified customer

    Submitted Feb 27 2019

    Question

    I can not find MSDS for Abs ab21218, ab32349, ab30743 on your website. Could you please provide me with these MSDS.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 26 2012

    Answer

    Thank you for contacting us.

    The MSDS files have been added to the online datasheets of these 3 antibodies (ab21218, ab32349, ab30743). You can find them now there or below:

    ab21218: https://www.abcam.com/ps/Products/21/ab21218/Msds/ab21218_WPNA_EN.pdf
    ab32349: https://www.abcam.com/ps/Products/32/ab32349/Msds/ab32349_WPNA_EN.pdf
    ab30743: https://www.abcam.com/ps/Products/30/ab30743/Msds/ab30743_WPNA_EN.pdf

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Jul 26 2012

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Dopamine D2 Receptor antibody - Neuronal Marker

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Read More

    Dr. Sophie Pezet

    Verified customer

    Submitted Mar 20 2012

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