Overview

  • Product name

    Anti-Dopamine D2 Receptor antibody
    See all Dopamine D2 Receptor primary antibodies
  • Description

    Rabbit polyclonal to Dopamine D2 Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chimpanzee, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human Dopamine D2 Receptor aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab97357)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Human brain; Human skeletal muscle; Rat Substantia nigra; Rat Dopaminergic nucleus.

Properties

Applications

Our Abpromise guarantee covers the use of ab85367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 50 kDa).

Target

  • Function

    This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
  • Involvement in disease

    Defects in DRD2 are associated with dystonia type 11 (DYT11) [MIM:159900]; also known as alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is characterized by involuntary lightning jerks and dystonic movements and postures alleviated by alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement, presence of myoclonus and response to alcohol are all variable.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • D(2) dopamine receptor antibody
    • D2 dopamine receptor antibody
    • D2DR antibody
    • D2R antibody
    • Dopamine D2 receptor antibody
    • Dopamine receptor D2 antibody
    • DRD 2 antibody
    • DRD2 antibody
    • DRD2_HUMAN antibody
    see all

Images

  • All lanes : Anti-Dopamine D2 Receptor antibody (ab85367) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 49 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes
  • All lanes : Anti-Dopamine D2 Receptor antibody (ab85367) at 1 µg/ml

    Lane 1 : Rat Substantia Nigra Lysate
    Lane 2 : Dopaminergic nucleus Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 49 kDa why is the actual band size different from the predicted?
    Additional bands at: 52 kDa (possible glycosylated form)


    Exposure time: 4 minutes

References

This product has been referenced in:

  • Lin CC  et al. Subchronic administration of aripiprazole improves fear extinction retrieval of Pavlovian conditioning paradigm in rats experiencing psychological trauma. Behav Brain Res 362:181-187 (2019). Read more (PubMed: 30610908) »
  • Beauvais G  et al. Efficient RNA interference-based knockdown of mutant torsinA reveals reversibility of PERK-eIF2a pathway dysregulation in DYT1 transgenic rats in vivo. Brain Res 1706:24-31 (2019). Read more (PubMed: 30366018) »
See all 7 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Flow Cytometry
Sample
Human Cell (AML3)
Permeabilization
Yes - 0.5% Triton
Gating Strategy
Live cells
Specification
AML3
Fixation
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 31 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (SH-SY5Y)
Permeabilization
Yes - TRITON X-100 (0'25%)
Specification
SH-SY5Y
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 02 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No

Dr. Sophie Pezet

Verified customer

Submitted Feb 08 2012

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