Anti-Dopamine Receptor D1 antibody (ab20066)
Key features and details
- Rabbit polyclonal to Dopamine Receptor D1
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Dopamine Receptor D1 antibody
See all Dopamine Receptor D1 primary antibodies -
Description
Rabbit polyclonal to Dopamine Receptor D1 -
Host species
Rabbit -
Specificity
Stains specific cells in various regions of PLP fixed Rat brain sections. This includes Medial septum, Nucleus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra reticulata, and the Ventral tegmental area. Cross reactivity: D1 Dopamine Receptor (9-21) 100%; D1 Dopamine Receptor 90%; D2 Dopamine Receptor (272-282) 0%; D2 Dopamine Receptor 0%; D3 Dopamine Receptor (2-10) 0%; D3 Dopamine Receptor 0; D4 Dopamine Receptor (176-185) 0%; D4 Dopamine Receptor 0%; D5 Dopamine Receptor (23-35) 0%; D5 Dopamine Receptor 0% -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide:
MDGTGLVVERDFS
, corresponding to amino acids 9-21 of Human Dopamine Receptor D1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: PBS, 1% BSA -
Concentration information loading...
-
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 49 kDa).
|
Notes |
---|
WB
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 49 kDa). |
Target
-
Function
Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. -
Tissue specificity
Detected in caudate, nucleus accumbens and in the olfactory tubercle. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14. - Information by UniProt
-
Database links
- Entrez Gene: 1812 Human
- Entrez Gene: 24316 Rat
- Omim: 126449 Human
- SwissProt: P21728 Human
- SwissProt: P18901 Rat
- Unigene: 2624 Human
- Unigene: 24039 Rat
-
Alternative names
- D(1A) dopamine receptor antibody
- D1A dopamine receptor antibody
- DADR antibody
see all
Images
-
All lanes : Anti-Dopamine Receptor D1 antibody (ab20066) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Rat Forebrain
Lane 3 : Rat Cortex Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa, 70 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (47)
ab20066 has been referenced in 47 publications.
- Sinani A et al. Early life stress influences basal ganglia dopamine receptors and novel object recognition of adolescent and adult rats. IBRO Neurosci Rep 12:342-354 (2022). PubMed: 35572456
- Lee GJ et al. Anterior Insular-nucleus Accumbens Pathway Controls Refeeding-induced Analgesia under Chronic Inflammatory Pain Condition. Neuroscience 495:58-73 (2022). PubMed: 35643248
- Wang S et al. Knockout of Dopamine D3 Receptor Gene Blocked Methamphetamine-Induced Distinct Changes of Dopaminergic and Glutamatergic Synapse in the Nucleus Accumbens Shell of Mice. Front Cell Neurosci 16:893190 (2022). PubMed: 35711471
- Chen LJ et al. Modulation of striatal glutamatergic, dopaminergic and cholinergic neurotransmission pathways concomitant with motor disturbance in rats with kaolin-induced hydrocephalus. Fluids Barriers CNS 19:95 (2022). PubMed: 36437472
- Lin L et al. Effects of Chemogenetic Inhibition of D1 or D2 Receptor-Containing Neurons of the Substantia Nigra and Striatum in Mice With Tourette Syndrome. Front Mol Neurosci 14:779436 (2021). PubMed: 34955745