Overview

  • Product name
    Anti-Dopamine Receptor D1 antibody
    See all Dopamine Receptor D1 primary antibodies
  • Description
    Rabbit polyclonal to Dopamine Receptor D1
  • Host species
    Rabbit
  • Specificity
    Stains specific cells in various regions of PLP fixed Rat brain sections. This includes Medial septum, Nucleus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra reticulata, and the Ventral tegmental area. Cross reactivity: D1 Dopamine Receptor (9-21) 100%; D1 Dopamine Receptor 90%; D2 Dopamine Receptor (272-282) 0%; D2 Dopamine Receptor 0%; D3 Dopamine Receptor (2-10) 0%; D3 Dopamine Receptor 0; D4 Dopamine Receptor (176-185) 0%; D4 Dopamine Receptor 0%; D5 Dopamine Receptor (23-35) 0%; D5 Dopamine Receptor 0%
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-FoFr, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Aplysia
  • Immunogen

    Synthetic peptide:

    MDGTGLVVERDFS

    , corresponding to amino acids 9-21 of Human Dopamine Receptor D1

  • Positive control
    • Whole Rat brain homogenate.

Properties

Applications

Our Abpromise guarantee covers the use of ab20066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 49 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 19006818
IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/5000.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
  • Tissue specificity
    Detected in caudate, nucleus accumbens and in the olfactory tubercle.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.
  • Information by UniProt
  • Database links
  • Alternative names
    • D(1A) dopamine receptor antibody
    • D1A dopamine receptor antibody
    • DADR antibody
    • Dopamine D1 receptor antibody
    • dopamine receptor D1 antibody
    • DR D1 antibody
    • DR D1A antibody
    • DRD 1 antibody
    • DRD 1A antibody
    • DRD1 antibody
    • DRD1_HUMAN antibody
    • DRD1A antibody
    see all

Images

  • All lanes : Anti-Dopamine Receptor D1 antibody (ab20066) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Rat Forebrain
    Lane 3 : Rat Cortex Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 45 kDa, 70 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 90 seconds
  • ab20066 staining Dopamine Receptor D1 in Mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde and blocked with 5% serum for 60 minutes at 23°C. The sample was incubated with primary antibody (1/600 in PBS + 5% Serum) at 4°C for 13 hours. An undiluted Alexa Fluor®555-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Lin L  et al. Cocaine- and amphetamine-regulated transcript (CART) is associated with dopamine and is protective against ischemic stroke. Mol Med Rep 18:3298-3304 (2018). Read more (PubMed: 30066844) »
  • Nobili A  et al. Dopamine neuronal loss contributes to memory and reward dysfunction in a model of Alzheimer's disease. Nat Commun 8:14727 (2017). WB . Read more (PubMed: 28367951) »
See all 15 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Application
Immunohistochemistry free floating
Sample
Zebra finch Tissue sections (Brain)
Specification
Brain

Luke Remage-Healey

Verified customer

Submitted Dec 18 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human leukemia cells)
Permeabilization
Yes - 0.25% Triton
Specification
Human leukemia cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Jake Ritchie

Verified customer

Submitted Sep 29 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
No
Specification
Brain
Blocking step
Normal donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 11 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
Antigen retrieval step
None
Sample
Aplysia Tissue sections (ganglia)
Specification
ganglia
Permeabilization
Yes - triton 0.2%
Fixative
Paraformaldehyde

Mrs. Francoise Geffroy

Verified customer

Submitted Oct 24 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: R°C
Antigen retrieval step
None - Buffer/Enzyme Used: citrate buffer pH6
Sample
Sheep Tissue sections (Sheep pituitary)
Specification
Sheep pituitary
Permeabilization
No
Fixative
Bouins

Abcam user community

Verified customer

Submitted Oct 10 2014

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C
Sample
Monkey Tissue sections (Brain (Frontal Cortex))
Specification
Brain (Frontal Cortex)
Permeabilization
Yes - 0.5% Triton X-100
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Apr 18 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Apr 24 2013

Answer

Thank you for your reply.

We do have a dopamine receptor D1 protein, ab90384, but I didn't recommend it because we haven't tested it as a positive control in WB.

https://www.abcam.com/Dopamine-Receptor-D1-protein-Active-ab90384.html

This is the full-length protein, but it will run higher than expected because it has an N-terminal c-myc tag.

I don't know if this would be of interest, but we are running a 3 for 2 special on all our proteins and peptides this month:

https://www.abcam.com/index.html?pageconfig=resource&rid=15079

Please let me know if the protocol suggestions do not improve your results.

Read More

Answer

Thank you for contacting us. I assume you are refering to the ICC/IF application? If not, please let me know. This application was added based on the publication PubMed: 19006818, and has not been tested in house in this application. The publication is: Goffin D et al. D1-like dopamine receptors regulate GABAA receptor function to modulate hippocampal neural progenitor cell proliferation. J Neurochem 107:964-75 (2008). ICC/IF; Mouse. PubMed: 19006818 Our recommended starting dilution in general for ICC/IF is 1/50 to 1/100, but details of the protocol that have worked for the customer who published the paper are not available to us, as we do not have access to this journal. Please checkthe paper or contact the authors of the paper directly for more details. We do not offer smaller sizes of the antibody for trial purposes as this is operationally not feasible, but we do guarantee the antibody for 6 months after the purchase for ICC/If with human, mouse and rat samples. We guarantee all our products for tested species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your inquiry. We would guarantee both ab20066 and ab40653 to work with rat samples in WB in accordance with our Abpromise. https://www.abcam.com/index.html?pageconfig=resource&rid=10372 I wouldn't be too concerned about the linker on the immunogen.  Most customers will choose an antibody based on the data we have on the datasheet.  These antibodies are more or less the same in terms of immunogen, testing information, purity, etc. We have specifically tested both in various regions of PLP fixed Rat brain sections. This includes Medial septum, Nucleus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra reticulata, and the Ventral tegmental area.  We have 2 references for ab20066: https://www.abcam.com/Dopamine-Receptor-D1-antibody-ab20066-references.html For ab40653, we do know of a rat-specific reference in WB. https://www.abcam.com/Dopamine-Receptor-D1-antibody-ab40653-references.html Essentially, we would guarantee either to work in the way you are testing, and these antibodies are essentially very similar. I hope this information helps.  Please contact us with any other questions.

Read More

1-10 of 11 Abreviews or Q&A

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