Product nameAnti-Dopamine Receptor D1 antibody
See all Dopamine Receptor D1 primary antibodies
DescriptionRabbit polyclonal to Dopamine Receptor D1
SpecificityStains specific cells in various regions of PLP fixed Rat brain sections. This includes Medial septum, Nucleus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra reticulata, and the Ventral tegmental area. Cross reactivity: D1 Dopamine Receptor (9-21) 100%; D1 Dopamine Receptor 90%; D2 Dopamine Receptor (272-282) 0%; D2 Dopamine Receptor 0%; D3 Dopamine Receptor (2-10) 0%; D3 Dopamine Receptor 0; D4 Dopamine Receptor (176-185) 0%; D4 Dopamine Receptor 0%; D5 Dopamine Receptor (23-35) 0%; D5 Dopamine Receptor 0%
Tested applicationsSuitable for: WB, ICC/IF, IHC-FoFr, IHC-P, ELISAmore details
Species reactivityReacts with: Mouse, Rat, Human, Aplysia
- Whole Rat brain homogenate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: PBS, 1% BSA
Our Abpromise guarantee covers the use of ab20066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 49 kDa).|
|ICC/IF||Use at an assay dependent concentration. PubMed: 19006818|
|IHC-FoFr||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
FunctionDopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
Tissue specificityDetected in caudate, nucleus accumbens and in the olfactory tubercle.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.
- Information by UniProt
- D(1A) dopamine receptor antibody
- D1A dopamine receptor antibody
- DADR antibody
All lanes : Anti-Dopamine Receptor D1 antibody (ab20066) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Rat Forebrain
Lane 3 : Rat Cortex Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa, 70 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
ab20066 staining Dopamine Receptor D1 in Mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde and blocked with 5% serum for 60 minutes at 23°C. The sample was incubated with primary antibody (1/600 in PBS + 5% Serum) at 4°C for 13 hours. An undiluted Alexa Fluor®555-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.
This product has been referenced in:
- Lin L et al. Cocaine- and amphetamine-regulated transcript (CART) is associated with dopamine and is protective against ischemic stroke. Mol Med Rep 18:3298-3304 (2018). Read more (PubMed: 30066844) »
- Frederick NM et al. Loss of the dystonia gene Thap1 leads to transcriptional deficits that converge on common pathogenic pathways in dystonic syndromes. Hum Mol Genet N/A:N/A (2018). Read more (PubMed: 30590536) »