Overview

  • Product name

    Anti-Dopamine Receptor D1 antibody
    See all Dopamine Receptor D1 primary antibodies
  • Description

    Rabbit polyclonal to Dopamine Receptor D1
  • Host species

    Rabbit
  • Specificity

    This antibody shows reactivity with: D1 Dopamine Receptor (9-21) 100%; D1 Dopamine Receptor 90%; D2 Dopamine Receptor (272-282) 0%; D2 Dopamine Receptor 0%; D3 Dopamine Receptor (2-10) 0%; D3 Dopamine Receptor 0%; D4 Dopamine Receptor (176-185) 0%; D4 Dopamine Receptor 0%; D5 Dopamine Receptor (23-35) 0%; and D5 Dopamine Receptor 0%.
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Pig, Macaque monkey, Rhesus monkey
  • Immunogen

    Synthetic peptide:

    MDGTGLVVERDFS

    linked to a carrier protein by a carboxyl terminal Cysteine residue linker. Sequence corresponds to amino acids 9-21 of Human Dopamine Receptor D1.

  • Positive control

    • ICC: PLP fixed rat brain sections. The antiserum has been found to stain specific cells in various regions, including the Medial septum, Nucleus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra reticulata, and the Ventral tegmental area. WB: whole rat brain homogenate.

Properties

  • Form

    Lyophilised:Reconstitute with 0.1ml of PBS which contains 10 mg/ml BSA.
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Constituent: Whole serum
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).Can be blocked with Dopamine Receptor D1 peptide (ab137968). With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
ICC/IF 1/5000.
IHC-P 1/500.

Target

Images

  • ab40653 (1:500) staining Dopamine Receptor D1 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic and membrane regions in the fibrous glial cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • All lanes : Anti-Dopamine Receptor D1 antibody (ab40653) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Rat Cortex Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 49 kDa
    Additional bands at: 42 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 90 seconds

References

This product has been referenced in:

  • Wang T  et al. Activation of dopamine D1 receptor decreased NLRP3-mediated inflammation in intracerebral hemorrhage mice. J Neuroinflammation 15:2 (2018). IHC ; Mouse . Read more (PubMed: 29301581) »
  • Zhao Y  et al. Memory retrieval in addiction: a role for miR-105-mediated regulation of D1 receptors in mPFC neurons projecting to the basolateral amygdala. BMC Biol 15:128 (2017). Read more (PubMed: 29282124) »
See all 7 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Rat brain striatum)
Specification
Rat brain striatum

Abcam user community

Verified customer

Submitted Apr 09 2019

Western blot

Inconclusive
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Sample
Mouse Tissue lysate - whole (hypothalamus and spine)
Specification
hypothalamus and spine
Blocking step
normal donkey serum/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Tatiana Novoselova

Verified customer

Submitted Jun 17 2013

Answer

Thank you for your patience.

I am happy to confirm that we were able to add the immunogen peptides for the antibody anti-Dopamine receptor 1 (ab40653) and the anti-Dopamine receptor 3 (ab40655).

The immunogen peptide for ab40653 can be found with the catalog number ab137968 https://www.abcam.com/index.html?datasheet=137968 (or use the following: https://www.abcam.com/index.html?datasheet=137968).

The immunogen peptide for ab40655 can be found with the catalog number ab137997 https://www.abcam.com/index.html?datasheet=137997 (or use the following: https://www.abcam.com/index.html?datasheet=137997).

I hope the peptides are useful for you. Please do not hesitate to contact us again, should you need any additional information or should youhave any other questions.

I wish you a good weekend!

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Answer

Thank you for your enquiry.

I am sorry to confirm that regrettablythese lot numbers for these antibodies are no longer available. These are very old lot numbers.

Should you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for your inquiry. We would guarantee both ab20066 and ab40653 to work with rat samples in WB in accordance with our Abpromise. https://www.abcam.com/index.html?pageconfig=resource&rid=10372 I wouldn't be too concerned about the linker on the immunogen.  Most customers will choose an antibody based on the data we have on the datasheet.  These antibodies are more or less the same in terms of immunogen, testing information, purity, etc. We have specifically tested both in various regions of PLP fixed Rat brain sections. This includes Medial septum, Nucleus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra reticulata, and the Ventral tegmental area.  We have 2 references for ab20066: https://www.abcam.com/Dopamine-Receptor-D1-antibody-ab20066-references.html For ab40653, we do know of a rat-specific reference in WB. https://www.abcam.com/Dopamine-Receptor-D1-antibody-ab40653-references.html Essentially, we would guarantee either to work in the way you are testing, and these antibodies are essentially very similar. I hope this information helps.  Please contact us with any other questions.

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