Anti-Dopamine Receptor D1 antibody (ab40653)


  • Product name
    Anti-Dopamine Receptor D1 antibody
    See all Dopamine Receptor D1 primary antibodies
  • Description
    Rabbit polyclonal to Dopamine Receptor D1
  • Host species
  • Specificity
    This antibody shows reactivity with: D1 Dopamine Receptor (9-21) 100%; D1 Dopamine Receptor 90%; D2 Dopamine Receptor (272-282) 0%; D2 Dopamine Receptor 0%; D3 Dopamine Receptor (2-10) 0%; D3 Dopamine Receptor 0%; D4 Dopamine Receptor (176-185) 0%; D4 Dopamine Receptor 0%; D5 Dopamine Receptor (23-35) 0%; and D5 Dopamine Receptor 0%.
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Pig, Macaque monkey, Rhesus monkey
  • Immunogen

    Synthetic peptide:


    linked to a carrier protein by a carboxyl terminal Cysteine residue linker. Sequence corresponds to amino acids 9-21 of Human Dopamine Receptor D1.

  • Positive control
    • ICC: PLP fixed rat brain sections. The antiserum has been found to stain specific cells in various regions, including the Medial septum, Nucleus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra reticulata, and the Ventral tegmental area. WB: whole rat brain homogenate.


  • Form
    Lyophilised:Reconstitute with 0.1ml of PBS which contains 10 mg/ml BSA.
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Constituent: Whole serum
  • Concentration information loading...
  • Purity
    Whole antiserum
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab40653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).Can be blocked with Dopamine Receptor D1 peptide (ab137968). With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
ICC/IF 1/5000.
IHC-P 1/500.



  • ab40653 (1:500) staining Dopamine Receptor D1 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic and membrane regions in the fibrous glial cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • All lanes : Anti-Dopamine Receptor D1 antibody (ab40653) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Rat Cortex Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 49 kDa
    Additional bands at: 42 kDa, 75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 90 seconds


This product has been referenced in:
  • Wang T  et al. Activation of dopamine D1 receptor decreased NLRP3-mediated inflammation in intracerebral hemorrhage mice. J Neuroinflammation 15:2 (2018). IHC ; Mouse . Read more (PubMed: 29301581) »
  • Zhao Y  et al. Memory retrieval in addiction: a role for miR-105-mediated regulation of D1 receptors in mPFC neurons projecting to the basolateral amygdala. BMC Biol 15:128 (2017). Read more (PubMed: 29282124) »

See all 7 Publications for this product

Customer reviews and Q&As

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Western blot

Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Mouse Tissue lysate - whole (hypothalamus and spine)
hypothalamus and spine
Blocking step
normal donkey serum/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Tatiana Novoselova

Verified customer

Submitted Jun 17 2013


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