Recombinant Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
![Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)](https://www.abcam.com/ps/products/81/ab81296/Images/ab81296-253826-anti-dopamine-receptor-d1-antibody-ep1560y-western-blot.jpg "Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Dopamine Receptor D1 antibody [EP1560Y]
See all Dopamine Receptor D1 primary antibodies -
Description
Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Guinea pig
-
Immunogen
Synthetic peptide within Human Dopamine Receptor D1 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P21728 -
Positive control
- WB: SH-SY5Y cell lysate, human fetal kidney, guinea pig brain, mouse and rat brain and kidney tissue lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1560Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
- Mouse kidney normal tissue lysate - total protein (ab29305)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
- Mouse kidney tissue lysate - total protein (0 days) (ab7261)
- Mouse kidney tissue lysate (14 days) (ab7262)
- Mouse kidney tissue lysate (7 days) (ab7263)
Applications
Our Abpromise guarantee covers the use of ab81296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 49 kDa. |
Target
-
Function
Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. -
Tissue specificity
Detected in caudate, nucleus accumbens and in the olfactory tubercle. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14. - Information by UniProt
-
Database links
- Entrez Gene: 1812 Human
- Entrez Gene: 13488 Mouse
- Entrez Gene: 24316 Rat
- Omim: 126449 Human
- SwissProt: P21728 Human
- SwissProt: Q61616 Mouse
- SwissProt: P18901 Rat
- Unigene: 2624 Human
see all -
Alternative names
- D(1A) dopamine receptor antibody
- D1A dopamine receptor antibody
- DADR antibody
see all
Images
-
Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)All lanes : Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/1000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat kidney tissue lysate
Lane 5 : Guinea pig brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1 (ab81296) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
-
![Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)](https://www.abcam.com/ps/products/81/ab81296/Images/ab81296-253825-anti-dopamine-receptor-d1-antibody-ep1560y-western-blot.jpg)
Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/5000 dilution (purified) + SH-SY5Y whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
-
![Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)](https://www.abcam.com/ps/products/81/ab81296/Images/ab81296-253824-anti-dopamine-receptor-d1-antibody-ep1560y-western-blot.jpg)
Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/1000 dilution (purified) + Human fetal kidney tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
-
![Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)](https://www.abcam.com/ps/products/81/ab81296/Images/ab81296-96501-anti-dopamine-receptor-d1-antibody-ep1560y-western-blot.jpg)
Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/20000 dilution (unpurified) + SH-SY5Y cell lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 49 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
![Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)](https://www.abcam.com/ps/products/81/ab81296/Images/ab81296-6-benefits-of-recombinant-antibodies.png)
Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
References (11)
ab81296 has been referenced in 11 publications.
- Zhang J et al. Voluntary Wheel Running Reverses Deficits in Social Behavior Induced by Chronic Social Defeat Stress in Mice: Involvement of the Dopamine System. Front Neurosci 13:256 (2019). PubMed: 31019446
- Yuan W et al. Preweaning paternal deprivation impacts parental responses to pups and alters the serum OT and CORT levels and OTR, V1AR, ER, D1R, D2R levels in relevant brain regions in adult mandarin voles. Neuroendocrinology N/A:N/A (2019). PubMed: 31256151
- Matsuyama N et al. The dopamine D1 receptor is expressed and induces CREB phosphorylation and MUC5AC expression in human airway epithelium. Respir Res 19:53 (2018). PubMed: 29606146
- Zuo F et al. Intrastriatal Transplantation of Human Neural Stem Cells Restores the Impaired Subventricular Zone in Parkinsonian Mice. Stem Cells 35:1519-1531 (2017). PubMed: 28328168
- Ren K et al. Striatal Distribution and Cytoarchitecture of Dopamine Receptor Subtype 1 and 2: Evidence from Double-Labeling Transgenic Mice. Front Neural Circuits 11:57 (2017). PubMed: 28860974
