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RecombinantRabMAb

Recombinant Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (11)

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Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
  • Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
  • Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
  • Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
  • Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-Dopamine Receptor D1 antibody [EP1560Y]
    See all Dopamine Receptor D1 primary antibodies
  • Description

    Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Guinea pig
  • Immunogen

    Synthetic peptide within Human Dopamine Receptor D1 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P21728

  • Positive control

    • WB: SH-SY5Y cell lysate, human fetal kidney, guinea pig brain, mouse and rat brain and kidney tissue lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1560Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Dopamine Receptors

Associated products

  • Alternative Versions

    • Anti-Dopamine Receptor D1 antibody [EP1560Y] - BSA and Azide free (ab170146)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Mouse kidney normal tissue lysate - total protein (ab29305)
    • Mouse brain tissue lysate - total protein (ab30151)
    • Mouse brain tissue lysate - total protein (0 days) (ab7188)
    • Mouse brain tissue lysate - total protein (14 days) (ab7189)
    • Mouse brain tissue lysate - total protein (7 days) (ab7190)
    • Mouse kidney tissue lysate - total protein (0 days) (ab7261)
    • Mouse kidney tissue lysate (14 days) (ab7262)
    • Mouse kidney tissue lysate (7 days) (ab7263)

Applications

Our Abpromise guarantee covers the use of ab81296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
    • Tissue specificity

      Detected in caudate, nucleus accumbens and in the olfactory tubercle.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.
    • Target information above from: UniProt accession P21728 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1812 Human
      • Entrez Gene: 13488 Mouse
      • Entrez Gene: 24316 Rat
      • Omim: 126449 Human
      • SwissProt: P21728 Human
      • SwissProt: Q61616 Mouse
      • SwissProt: P18901 Rat
      • Unigene: 2624 Human
      • Unigene: 54161 Mouse
      • Unigene: 24039 Rat
      see all
    • Alternative names

      • D(1A) dopamine receptor antibody
      • D1A dopamine receptor antibody
      • DADR antibody
      • Dopamine D1 receptor antibody
      • dopamine receptor D1 antibody
      • DR D1 antibody
      • DR D1A antibody
      • DRD 1 antibody
      • DRD 1A antibody
      • DRD1 antibody
      • DRD1_HUMAN antibody
      • DRD1A antibody
      see all

    Images

    • Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
      Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
      All lanes : Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/1000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse kidney tissue lysate
      Lane 3 : Rat brain tissue lysate
      Lane 4 : Rat kidney tissue lysate
      Lane 5 : Guinea pig brain tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1 (ab81296) at 1/20000 dilution

      Predicted band size: 49 kDa
      Observed band size: 75 kDa
      why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM /TBST.

    • Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
      Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
      Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/5000 dilution (purified) + SH-SY5Y whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 49 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM /TBST.

    • Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
      Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
      Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/1000 dilution (purified) + Human fetal kidney tissue lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 49 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM /TBST.

    • Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
      Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
      Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/20000 dilution (unpurified) + SH-SY5Y cell lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 49 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?

    • Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
      Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (11)

    Publishing research using ab81296? Please let us know so that we can cite the reference in this datasheet.

    ab81296 has been referenced in 11 publications.

    • Zhang J  et al. Voluntary Wheel Running Reverses Deficits in Social Behavior Induced by Chronic Social Defeat Stress in Mice: Involvement of the Dopamine System. Front Neurosci 13:256 (2019). PubMed: 31019446
    • Yuan W  et al. Preweaning paternal deprivation impacts parental responses to pups and alters the serum OT and CORT levels and OTR, V1AR, ER, D1R, D2R levels in relevant brain regions in adult mandarin voles. Neuroendocrinology N/A:N/A (2019). PubMed: 31256151
    • Matsuyama N  et al. The dopamine D1 receptor is expressed and induces CREB phosphorylation and MUC5AC expression in human airway epithelium. Respir Res 19:53 (2018). PubMed: 29606146
    • Zuo F  et al. Intrastriatal Transplantation of Human Neural Stem Cells Restores the Impaired Subventricular Zone in Parkinsonian Mice. Stem Cells 35:1519-1531 (2017). PubMed: 28328168
    • Ren K  et al. Striatal Distribution and Cytoarchitecture of Dopamine Receptor Subtype 1 and 2: Evidence from Double-Labeling Transgenic Mice. Front Neural Circuits 11:57 (2017). PubMed: 28860974
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Dopamine Receptor D1 antibody [EP1560Y]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (striatum)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citric acid
    Permeabilization
    No
    Specification
    striatum
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 28 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Dopamine Receptor D1 antibody [EP1560Y]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Dec 14 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Dopamine Receptor D1 antibody [EP1560Y]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Dec 14 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-Dopamine Receptor D1 antibody [EP1560Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Zebra finch Tissue sections (Area X (striatum) brain tissue)
    Permeabilization
    No
    Specification
    Area X (striatum) brain tissue
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. Jonnathan Singh

    Verified customer

    Submitted Jun 24 2015

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