Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)
- Datasheet
- Specific References (8)
- Protocols
Overview
-
Product nameAnti-Dopamine Receptor D1 antibody [EP1560Y]
See all Dopamine Receptor D1 primary antibodies -
DescriptionRabbit monoclonal [EP1560Y] to Dopamine Receptor D1
-
Host speciesRabbit
-
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivityReacts with: Mouse, Rat, Guinea pig, Human
-
Immunogen
Synthetic peptide within Human Dopamine Receptor D1 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P21728 -
Positive control
- WB: SH-SY5Y cell lysate, human fetal kidney, guinea pig brain, mouse and rat brain and kidney tissue lysates.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
-
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
PurityProtein A purified
-
ClonalityMonoclonal
-
Clone numberEP1560Y
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
- Mouse kidney normal tissue lysate - total protein (ab29305)
- Rat brain normal tissue lysate - total protein (ab29475)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
- Mouse kidney tissue lysate - total protein (0 days) (ab7261)
- Mouse kidney tissue lysate (14 days) (ab7262)
- Mouse kidney tissue lysate (7 days) (ab7263)
Applications
Our Abpromise guarantee covers the use of ab81296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 49 kDa. |
Target
-
FunctionDopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
-
Tissue specificityDetected in caudate, nucleus accumbens and in the olfactory tubercle.
-
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
-
Cellular localizationCell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.
- Information by UniProt
-
Database links
- Entrez Gene: 1812 Human
- Entrez Gene: 13488 Mouse
- Entrez Gene: 24316 Rat
- Omim: 126449 Human
- SwissProt: P21728 Human
- SwissProt: Q61616 Mouse
- SwissProt: P18901 Rat
- Unigene: 2624 Human
see all -
Alternative names
- D(1A) dopamine receptor antibody
- D1A dopamine receptor antibody
- DADR antibody
see all
Images
-
![Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)](https://www.abcam.com/ps/products/81/ab81296/Images/ab81296-5-ab81296WB3.jpg)
Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)All lanes : Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/1000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat kidney tissue lysate
Lane 5 : Guinea pig brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit monoclonal [EP1560Y] to Dopamine Receptor D1 (ab81296) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 75 kDa (why is the actual band size different from the predicted?)Blocking and dilution buffer: 5% NFDM /TBST.
-
![Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)](https://www.abcam.com/ps/products/81/ab81296/Images/ab81296-4-ab81296WB2.jpg)
Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/5000 dilution (purified) + SH-SY5Y whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 75 kDa (why is the actual band size different from the predicted?)Blocking and dilution buffer: 5% NFDM /TBST.
-
![Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)](https://www.abcam.com/ps/products/81/ab81296/Images/ab81296-3-ab81296WB1.jpg)
Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/1000 dilution (purified) + Human fetal kidney tissue lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 75 kDa (why is the actual band size different from the predicted?)Blocking and dilution buffer: 5% NFDM /TBST.
-
![Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)](https://www.abcam.com/ps/datasheet/images/81/ab81296/Dopamine-Receptor-D1-Primary-antibodies-ab81296-1.JPG)
Western blot - Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296)Anti-Dopamine Receptor D1 antibody [EP1560Y] (ab81296) at 1/20000 dilution (unpurified) + SH-SY5Y cell lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 49 kDa
Observed band size: 75 kDa (why is the actual band size different from the predicted?)
Protocols
References
This product has been referenced in:
- Matsuyama N et al. The dopamine D1 receptor is expressed and induces CREB phosphorylation and MUC5AC expression in human airway epithelium. Respir Res 19:53 (2018). Read more (PubMed: 29606146) »
- Ren K et al. Striatal Distribution and Cytoarchitecture of Dopamine Receptor Subtype 1 and 2: Evidence from Double-Labeling Transgenic Mice. Front Neural Circuits 11:57 (2017). Read more (PubMed: 28860974) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"