Overview

  • Product name

    Anti-Dopamine Receptor D1 antibody [SG2-D1a]
    See all Dopamine Receptor D1 primary antibodies
  • Description

    Mouse monoclonal [SG2-D1a] to Dopamine Receptor D1
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, IHC (PFA fixed)more details
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep
    Does not react with: Rabbit, Cow, Dog, Human, Pig
  • Immunogen

    Recombinant fragment corresponding to Rat Dopamine Receptor D1 (C terminal).

  • Positive control

    • Mouse brain lysate; rat caudate tissue.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    SG2-D1a
  • Isotype

    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
WB 1/250 - 1/500. Predicted molecular weight: 49 kDa.
IHC (PFA fixed) Use at an assay dependent concentration.

Target

  • Function

    Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
  • Tissue specificity

    Detected in caudate, nucleus accumbens and in the olfactory tubercle.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.
  • Information by UniProt
  • Database links

  • Alternative names

    • D(1A) dopamine receptor antibody
    • D1A dopamine receptor antibody
    • DADR antibody
    • Dopamine D1 receptor antibody
    • dopamine receptor D1 antibody
    • DR D1 antibody
    • DR D1A antibody
    • DRD 1 antibody
    • DRD 1A antibody
    • DRD1 antibody
    • DRD1_HUMAN antibody
    • DRD1A antibody
    see all

Images

  • Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded rat cordical tissue sections, labelling Dopamine Receptor D1 with ab78021 at a dilution of 1/500 incubated for 2 hours at 21°C in TBS, BSA & azide diluent. Heat mediated antigen retrival was done via citric acid. Blocking was with 1% BSA incubated for 10 minutes at 21°C. Secondary used was a Goat anti-mouse poyclonal biotin conjugate at 1/300. Image of subventricular region of striatum shows appropriate positivitiy.

    See Abreview

  • Anti-Dopamine Receptor D1 antibody [SG2-D1a] (ab78021) at 2 µg/ml + Brain (Mouse) Tissue Lysate at 20 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 100 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes
  • IHC image of ab78021 staining in human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab78021, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Kubickova J  et al. Haloperidol Affects Plasticity of Differentiated NG-108 Cells Through s1R/IP3R1 Complex. Cell Mol Neurobiol 38:181-194 (2018). Read more (PubMed: 28786032) »
  • Lencesova L  et al. Disruption of dopamine D1/D2 receptor complex is involved in the function of haloperidol in cardiac H9c2 cells. Life Sci 191:186-194 (2017). ICC/IF . Read more (PubMed: 29054453) »
See all 3 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (brain)
Antigen retrieval step
Heat mediated
Permeabilization
Yes - TBS+0.1% Triton for 6min
Specification
brain
Blocking step
CAS-Block 008120, Thermofisher Scientific as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 24 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Sep 21 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Sep 21 2015

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (stacking gel: 4%, resolving gel: 5-13%)
Sample
Rat Tissue lysate - whole (hippocampi total membrane fraction)
Specification
hippocampi total membrane fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Ms. Sunetra Jitkar

Verified customer

Submitted Feb 19 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Tissue sections (Hippocampus)
Specification
Hippocampus
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 10 2014

Question
Answer

Thank you for confirming that information. I have organised for ab98790 to be sent out to you (Order number xxxxx, Purchase order numberFOCR xxxxx). As we have the Easter bank holidays this will unfortunately only reach you on Wednesday next week.

Hopefully, this new secondary antibody will help the situation. However, if you find the same all over staining please do let me know and we'll take it one reagent at a time to isolate the step which is causing the problems.

I look forward to hearing how you get on.

Read More

Answer

Thank you for getting back to me.

I can arrange for a different secondary antibody to be sent to you. In order to organise this I will need the order numbers of ab78021 and ab97040, or the approximate delivery date and delivery address.

Once I have this information I will look into an appropriate alternative to try.

I look forward to receiving your reply.

Read More

Answer

Thank you for contacting us.


As discussed over the phone, the dilution factors stated on the datasheet are recommended starting points only. Depending on the sample you are using and the protocol being used you may need to increase or decrease the dilution for optimum results. In this case it seems like you may need to increase the dilution further (from 1/500 to 1/1000 would be a good starting point as discussed over the phone).


Additionally, we have used BSA blocking to characterise this antibody. Switching from milk blocking to BSA can sometimes lead to significant reduction in non-specificity seen, as can be seen on the western blot presented on the datasheet of

https://www.abcam.com/ab9385.

I would suggest rying with 5% BSA, with 1% BSA in the antibody diluent.


If you would like me to take a closer look at the protocol you are using to make any further suggestions please fill our the questionnaire I have attached to this email. If you could include an image of the results you have been observing that will help me to understand the situation more fully.


I hope this information has been of help. I look forward to receiving your reply.

Read More

Answer

Thank you for contacting us. The protocol is, for the most part, standard. The instruction to not boil the samples is referring to the tendency of multi-pass membrane proteins to aggregate if boiled. To denature your samples before loading into the gel, heat the samples to no more than 70C for 10 minutes instead of boiling. This will be sufficient to denature the samples and will minimize the aggregation. The blocking buffer and diluent we recommend is 5% BSA in PBS. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

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