Product nameAnti-Dopamine Receptor D1 antibody [SG2-D1a]
See all Dopamine Receptor D1 primary antibodies
DescriptionMouse monoclonal [SG2-D1a] to Dopamine Receptor D1
Tested applicationsSuitable for: IHC-P, WB, IHC (PFA fixed)more details
Species reactivityReacts with: Mouse, Rat, Sheep
Does not react with: Rabbit, Cow, Dog, Human, Pig
Recombinant fragment corresponding to Rat Dopamine Receptor D1 (C terminal).
- Mouse brain lysate; rat caudate tissue.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab78021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250 - 1/500. Predicted molecular weight: 49 kDa.|
|IHC (PFA fixed)||Use at an assay dependent concentration.|
FunctionDopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
Tissue specificityDetected in caudate, nucleus accumbens and in the olfactory tubercle.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.
- Information by UniProt
- D(1A) dopamine receptor antibody
- D1A dopamine receptor antibody
- DADR antibody
Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded rat cordical tissue sections, labelling Dopamine Receptor D1 with ab78021 at a dilution of 1/500 incubated for 2 hours at 21°C in TBS, BSA & azide diluent. Heat mediated antigen retrival was done via citric acid. Blocking was with 1% BSA incubated for 10 minutes at 21°C. Secondary used was a Goat anti-mouse poyclonal biotin conjugate at 1/300. Image of subventricular region of striatum shows appropriate positivitiy.
Anti-Dopamine Receptor D1 antibody [SG2-D1a] (ab78021) at 2 µg/ml + Brain (Mouse) Tissue Lysate at 20 µg
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
IHC image of ab78021 staining in human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab78021, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Kubickova J et al. Haloperidol Affects Plasticity of Differentiated NG-108 Cells Through s1R/IP3R1 Complex. Cell Mol Neurobiol 38:181-194 (2018). Read more (PubMed: 28786032) »
- Lencesova L et al. Disruption of dopamine D1/D2 receptor complex is involved in the function of haloperidol in cardiac H9c2 cells. Life Sci 191:186-194 (2017). ICC/IF . Read more (PubMed: 29054453) »