Overview

  • Product name

    Anti-Dopamine Receptor D3/DRD3 antibody
    See all Dopamine Receptor D3/DRD3 primary antibodies
  • Description

    Rabbit polyclonal to Dopamine Receptor D3/DRD3
  • Host species

    Rabbit
  • Specificity

    D3 (2-10) 100% D3 80% Does not cross-react with other Dopamine Receptors (D1, D2, D4, D5)
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to Dopamine Receptor D3/DRD3 aa 2-10.
    Sequence:

    ASLSQLSSH-C


    (Peptide available as ab137997)

  • Positive control

    • Whole rat brain homogenate This antibody gave a positive result when used in the following methanol fixed cell lines: PC12
  • General notes

     This product was previously labelled as Dopamine Receptor D3

     

Properties

  • Form

    Liquid
  • Storage instructions

    Store at +4°C short term (1-2 weeks) (add antibacterial agent). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Constituent: Whole serum
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/5000. Stains specific cells in various regions of PLP-fixed rat brain sections including dedritc labelling in the molecular layers as well as the plasma membranes of neurons in layer 5 or cortex and the glomeruli of the olfactory bulb.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 44 kDa). Blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.

Target

  • Function

    This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation.
  • Tissue specificity

    Brain.
  • Involvement in disease

    Genetic variation in DRD3 is associated with essential tremor hereditary type 1 (ETM1) [MIM:190300]. ETM1 is the most common movement disorder. The main feature is postural tremor of the arms. Head, legs, trunk, voice, jaw, and facial muscles also may be involved. The condition can be aggravated by emotions, hunger, fatigue and temperature extremes, and may cause a functional disability or even incapacitation. Inheritance is autosomal dominant.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    Phosphorylated by GRK4 (GRK4-alpha and GRK4-gamma).
  • Cellular localization

    Cell membrane. Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3dopamine receptor antibody
    • D antibody
    • D(3) dopamine receptor antibody
    • Dopamine D3 receptor antibody
    • Dopamine receptor D3 antibody
    • DRD3 antibody
    • DRD3_HUMAN antibody
    • ETM1 antibody
    • FET1 antibody
    see all

Images

  • ab40655 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab40655 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab40655 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Application
Flow Cytometry
Sample
Human Cell (THP1 cells)
Permeabilization
Yes - 0.5% Triton X-100
Gating Strategy
Live cells
Specification
THP1 cells
Fixation
Formaldehyde

Abcam user community

Verified customer

Submitted Dec 19 2017

Answer

All our antibodies are for research purposes only and not for diagnostic or therapeutic ends. We therefore mainly test our products in in vitro application such as WB and IHC. All four antibodies mentioned have not been tested in vivo however this does not mean that they will not be suitable provided that this in vivo testing is not in humans and is in mouse or rat.

If you wanted to do in vivo testing, please note that Anti-Dopamine Transporter antibody (ab111468) and Anti-Dopamine Transporter antibody (ab111468) have 0.02% sodium azide in them as a preservative and that this would have to be removed before in vivo testing in rats as it could be harmful to them.

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Answer

Thank you for your patience.

I am happy to confirm that we were able to add the immunogen peptides for the antibody anti-Dopamine receptor 1 (ab40653) and the anti-Dopamine receptor 3 (ab40655).

The immunogen peptide for ab40653 can be found with the catalog number ab137968 https://www.abcam.com/index.html?datasheet=137968 (or use the following: https://www.abcam.com/index.html?datasheet=137968).

The immunogen peptide for ab40655 can be found with the catalog number ab137997 https://www.abcam.com/index.html?datasheet=137997 (or use the following: https://www.abcam.com/index.html?datasheet=137997).

I hope the peptides are useful for you. Please do not hesitate to contact us again, should you need any additional information or should youhave any other questions.

I wish you a good weekend!

Read More

Answer

Thank you for contacting us with your questions, and for yourpatience while I have been in touch with the lab regarding your enquiry.

I have confirmed with the lab that this is taken from the human DRD3 amino acid sequence, which can be found toward the bottom of the UniProt page below:

http://www.uniprot.org/uniprot/P35462

The immunogen corresponds to the N-terminalamino acids 2-10including an extra cysteine.

I hope this information will be useful but if you have any further questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

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Answer

Thank you for your enquiry.

I am sorry to confirm that regrettablythese lot numbers for these antibodies are no longer available. These are very old lot numbers.

Should you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for contacting us.
We currently haven't tested this antibody in immunohistochemistry so we do not know how this antibody behaves in IHC. The DDR3 receptors localizes either in cytoplasm or membrane so the staining should be cytoplasmic or membranous rather than nuclear. There are many publication that actually evidenced the expression of the protein in cytoplasmic region. It will be worth to check the protocol they have been using.
http://neurotalk.psychcentral.com/archive/index.php/t-20456.html
http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2006.04241.x/full
I look forward to hearing from you soon. Please do not hesitate to contact us if you have any question.

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Application
Flow Cytometry
Sample
Rat Cell (Spleen cells)
Permeabilization
No
Gating Strategy
Mononuclear Cells
Specification
Spleen cells
Preparation
Cell harvesting/tissue preparation method: Spleen cell suspensions were obtained by gently grinding tissue into RPMI 1640 culture medium (Sigma-Aldrich, Steinheim, Germany) under sterile conditions. Mononuclear cells were separated by Ficoll–Hypaque density gradient (1.083 g/ml) centrifugation. The mononuclear cells concentrated at the surface were collected and washed twice in RPMI 1640 medium. After cell counting, 1 x 10 6 cells/sample were stained with the corresponding antibodies.
Sample buffer: RPMI 1640 culture medium (Sigma-Aldrich, Steinheim, Germany)
Fixation
FACSlysing BD

Abcam user community

Verified customer

Submitted Jun 02 2011

Answer

Thank you for your enquiry. The immunogen, ASLSQLSSH, is from the N terminus, as shown in the alignment at the following link: http://www.ebi.ac.uk/cgi-bin/clustalw/result?tool=clustalw&jobid=clustalw-20070926-21493553&poll=yes This is an extracellular region of the receptor. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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