Anti-Dopamine Receptor D3/DRD3 antibody (ab42114)
Key features and details
- Rabbit polyclonal to Dopamine Receptor D3/DRD3
- Suitable for: ELISA, Dot blot, ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Dopamine Receptor D3/DRD3 antibody
See all Dopamine Receptor D3/DRD3 primary antibodies -
Description
Rabbit polyclonal to Dopamine Receptor D3/DRD3 -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, Dot blot, ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Synthetic peptide corresponding to Dopamine Receptor D3/DRD3.
Sequence:CQACHVSPELYRATTWGY
(Peptide available asab128688) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab42114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| Dot blot |
Use at an assay dependent concentration.
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| IP |
Use at an assay dependent concentration.
2 µl of this antibody will immunoprecpitate 65-80% dopamine recombinant D3 receptor protein from COS1 cells. |
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| WB | (1) |
1/1000 - 1/15000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Can be blocked with Dopamine Receptor D3/DRD3 peptide (ab128688).
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| Notes |
|---|
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ELISA
Use at an assay dependent concentration. |
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Dot blot
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 5 µg/ml. |
|
IP
Use at an assay dependent concentration. 2 µl of this antibody will immunoprecpitate 65-80% dopamine recombinant D3 receptor protein from COS1 cells. |
|
WB
1/1000 - 1/15000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Can be blocked with Dopamine Receptor D3/DRD3 peptide (ab128688). |
Target
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Function
This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation. -
Tissue specificity
Brain. -
Involvement in disease
Genetic variation in DRD3 is associated with essential tremor hereditary type 1 (ETM1) [MIM:190300]. ETM1 is the most common movement disorder. The main feature is postural tremor of the arms. Head, legs, trunk, voice, jaw, and facial muscles also may be involved. The condition can be aggravated by emotions, hunger, fatigue and temperature extremes, and may cause a functional disability or even incapacitation. Inheritance is autosomal dominant. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsPhosphorylated by GRK4 (GRK4-alpha and GRK4-gamma). -
Cellular localization
Cell membrane. Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells. - Information by UniProt
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Database links
- Entrez Gene: 1814 Human
- Entrez Gene: 13490 Mouse
- Entrez Gene: 29238 Rat
- Omim: 126451 Human
- SwissProt: P35462 Human
- SwissProt: P30728 Mouse
- SwissProt: P19020 Rat
- Unigene: 121478 Human
see all -
Alternative names
- 3dopamine receptor antibody
- D antibody
- D(3) dopamine receptor antibody
see all
Images
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Western blot - Anti-Dopamine Receptor D3/DRD3 antibody (ab42114)Anti-Dopamine Receptor D3/DRD3 antibody (ab42114) at 1/750 dilution + PC-D3R sample
Predicted band size: 50 kDa
Observed band size: 50 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Dopamine Receptor D3/DRD3 antibody (ab42114)ICC/IF image of ab42114 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42114, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (12)
ab42114 has been referenced in 12 publications.
- Williford SE et al. Novel dopamine receptor 3 antagonists inhibit the growth of primary and temozolomide resistant glioblastoma cells. PLoS One 16:e0250649 (2021). PubMed: 33945569
- Reyes-Resina I et al. Expression of Melatonin and Dopamine D3 Receptor Heteromers in Eye Ciliary Body Epithelial Cells and Negative Correlation with Ocular Hypertension. Cells 9:N/A (2020). PubMed: 31936298
- Huang J et al. Involvement of dopamine D3 receptor and dopamine transporter in methamphetamine-induced behavioral sensitization in tree shrews. Brain Behav 10:e01533 (2020). PubMed: 31943832
- Haruyama N et al. 8-Oxoguanine accumulation in aged female brain impairs neurogenesis in the dentate gyrus and major island of Calleja, causing sexually dimorphic phenotypes. Prog Neurobiol N/A:101613 (2019). PubMed: 31026482
- Jiang L et al. Brain Renin-Angiotensin System Blockade Attenuates Methamphetamine-Induced Hyperlocomotion and Neurotoxicity. Neurotherapeutics 15:500-510 (2018). PubMed: 29464572
