Overview

  • Product name

    Anti-Dopamine Receptor D3/DRD3 antibody [EPR10148]
    See all Dopamine Receptor D3/DRD3 primary antibodies
  • Description

    Rabbit monoclonal [EPR10148] to Dopamine Receptor D3/DRD3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human Dopamine Receptor D3/DRD3.
    Database link: P35462

  • Positive control

    • SH-SY5Y, Human fetal brain and Mouse brain lysates.
  • General notes

     

     

     This product was previously labelled as Dopamine Receptor D3

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10148
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation.
    • Tissue specificity

      Brain.
    • Involvement in disease

      Genetic variation in DRD3 is associated with essential tremor hereditary type 1 (ETM1) [MIM:190300]. ETM1 is the most common movement disorder. The main feature is postural tremor of the arms. Head, legs, trunk, voice, jaw, and facial muscles also may be involved. The condition can be aggravated by emotions, hunger, fatigue and temperature extremes, and may cause a functional disability or even incapacitation. Inheritance is autosomal dominant.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Post-translational
      modifications

      Phosphorylated by GRK4 (GRK4-alpha and GRK4-gamma).
    • Cellular localization

      Cell membrane. Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells.
    • Information by UniProt
    • Database links

    • Alternative names

      • 3dopamine receptor antibody
      • D antibody
      • D(3) dopamine receptor antibody
      • Dopamine D3 receptor antibody
      • Dopamine receptor D3 antibody
      • DRD3 antibody
      • DRD3_HUMAN antibody
      • ETM1 antibody
      • FET1 antibody
      see all

    Images

    • All lanes : Anti-Dopamine Receptor D3/DRD3 antibody [EPR10148] (ab155098) at 1/1000 dilution

      Lane 1 : SH-SY5Y lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : Mouse brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 44 kDa

    References

    This product has been referenced in:

    • Seo JH & Kuzhikandathil EV Dopamine D3 Receptor Mediates Preadolescent Stress-Induced Adult Psychiatric Disorders. PLoS One 10:e0143908 (2015). Read more (PubMed: 26619275) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde

    Mr. Carl Hobbs

    Verified customer

    Submitted Dec 14 2015

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde

    Mr. Carl Hobbs

    Verified customer

    Submitted Dec 14 2015

    This product is known to not work in this application or species.
    Application
    Flow Cytometry
    Sample
    Human Cell (Colon)
    Permeabilization
    Yes - 0.1% TritonX-100
    Specification
    Colon
    Preparation
    Cell harvesting/tissue preparation method: 1mM EDTA Trypsin
    Sample buffer: 2% FBS/DPBS
    Fixation
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jun 24 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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