Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10148] to Dopamine Receptor D3/DRD3 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-Dopamine Receptor D3/DRD3 antibody [EPR10148] - BSA and Azide free
See all Dopamine Receptor D3/DRD3 primary antibodies
DescriptionRabbit monoclonal [EPR10148] to Dopamine Receptor D3/DRD3 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Dopamine Receptor D3/DRD3. The exact sequence is proprietary.
ab240131 is the carrier-free version of ab155098 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab240131 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Dopamine Receptor D3
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab240131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 44 kDa.|
FunctionThis is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation.
Involvement in diseaseGenetic variation in DRD3 is associated with essential tremor hereditary type 1 (ETM1) [MIM:190300]. ETM1 is the most common movement disorder. The main feature is postural tremor of the arms. Head, legs, trunk, voice, jaw, and facial muscles also may be involved. The condition can be aggravated by emotions, hunger, fatigue and temperature extremes, and may cause a functional disability or even incapacitation. Inheritance is autosomal dominant.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
modificationsPhosphorylated by GRK4 (GRK4-alpha and GRK4-gamma).
Cellular localizationCell membrane. Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells.
- Information by UniProt
- 3dopamine receptor antibody
- D antibody
- D(3) dopamine receptor antibody
All lanes : Anti-Dopamine Receptor D3/DRD3 antibody [EPR10148] (ab155098) at 1/1000 dilution
Lane 1 : SH-SY5Y lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Mouse brain lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 44 kDa
This data was developed using ab155098, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240131 has not yet been referenced specifically in any publications.