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Dopamine Receptor D3/DRD3 peptide (ab128688)

Reviews (1) Submit a question References (2)

Key features and details

  • Purity: = 88% HPLC
  • Suitable for: WB, Blocking

Description

  • Product name

    Dopamine Receptor D3/DRD3 peptide

  • Purity

    = 88 % HPLC.
    Purified on a Reversed-Phase HPLC column and quality and characterized by mass spec. The purity of these peptides is checked by analytical HPLC and calculated by considering the area under the peptide peak as a percentage of areas under all peaks.

  • Animal free

    No

  • Nature

    Synthetic

Associated products

Specifications

Our Abpromise guarantee covers the use of ab128688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Blocking - Blocking peptide for Anti-Dopamine Receptor D3/DRD3 antibody (ab42114)

  • Form

    Liquid

  • Additional notes

     This product was previously labelled as Dopamine Receptor D3

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

General Info

  • Alternative names

    • 3dopamine receptor
    • D
    • D(3) dopamine receptor
    • Dopamine D3 receptor
    • Dopamine receptor D3
    • DRD3
    • DRD3_HUMAN
    • ETM1
    • FET1
    see all

  • Function

    This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation.

  • Tissue specificity

    Brain.

  • Involvement in disease

    Genetic variation in DRD3 is associated with essential tremor hereditary type 1 (ETM1) [MIM:190300]. ETM1 is the most common movement disorder. The main feature is postural tremor of the arms. Head, legs, trunk, voice, jaw, and facial muscles also may be involved. The condition can be aggravated by emotions, hunger, fatigue and temperature extremes, and may cause a functional disability or even incapacitation. Inheritance is autosomal dominant.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.

  • Post-translational
    modifications

    Phosphorylated by GRK4 (GRK4-alpha and GRK4-gamma).

  • Cellular localization

    Cell membrane. Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (2)

Publishing research using ab128688? Please let us know so that we can cite the reference in this datasheet.

ab128688 has been referenced in 2 publications.

  • Kilic T  et al. Mining the Potential of Label-Free Biosensors for In Vitro Antipsychotic Drug Screening. Biosensors (Basel) 8:N/A (2018). PubMed: 29315269
  • Ustione A & Piston DW Dopamine synthesis and D3 receptor activation in pancreatic ß-cells regulates insulin secretion and intracellular [Ca(2+)] oscillations. Mol Endocrinol 26:1928-40 (2012). Blocking . PubMed: 22918877

Customer reviews and Q&As

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Western blot Abreview for Dopamine Receptor D3 peptide

 Excellent
Abreviews
abreview image
Application
Western blot

Review text: This peptide was used to neutralize the D3 polyclonal antibody (ab42114).
Sample: Mouse Cell lysate - other (pancreatic islet lysate)
Loading amount: 18 µg
Specification: pancreatic islet lysate
Gel Running Conditions: Reduced Denaturing (10% gel)
Blocking step: BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details
Dilution: 1/2500
Incubation time: 1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T + 0.5%BSA

Primary antibody (in addition to 'Dopamine Receptor D3 peptide')
Primary antibody: Abcam primary antibody: Anti-Dopamine Receptor D3 antibody (ab42114)
Dilution: 1/750

Secondary antibody
Name: Non-Abcam antibody was used: Donkey anti-Rabbit IgG Antibody, AP182P
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/100000

Detection
Detection method: ECLprime
Exposure: 10 minute(s) and 0 second(s)
Bands: Specific: 47 kDa Non-specific: 15, 25, 31 kDa

Additional data
PubMed ID: 22918877: View
Additional Notes: The neutralization of the D3 antibody with the D3 peptide resulted in the absence of just the 47kDa specific band, that was previously observed. All the other bands were then considered non-specific.
Lane1: membrane fraction
Lane 2: cytosolic fracion
Lane 3: nuclear fraction
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 28 2013

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