Product nameAnti-Dopamine Transporter antibody [6V-23-23]
See all Dopamine Transporter primary antibodies
DescriptionMouse monoclonal [6V-23-23] to Dopamine Transporter
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
Does not react with: Human
Synthetic peptide corresponding to Rat Dopamine Transporter (N terminal).
- In western blot, this antibody gave a positive signal in mouse and rat forebrain tissue lysates, and in rat brain striatal lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab128848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).|
FunctionAmine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.
Involvement in diseaseDefects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI) [MIM:613135]. It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs.
Sequence similaritiesBelongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.
- Information by UniProt
- DA transporter antibody
- DAT 1 antibody
- DAT antibody
All lanes : Anti-Dopamine Transporter antibody [6V-23-23] (ab128848) at 5 µg/ml
Lane 1 : Forebrain (Mouse) Tissue Lysate
Lane 2 : Forebrain (Rat) Tissue Lysate
Lane 3 : Cerebellum (Rat) Tissue Lysate
Lane 4 : Brain (Rat) Striatal Tissue Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Additional bands at: 18 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab128848 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.
Rat cerebellum (lane 3) was included as a negative control. This tissue does not express dopamine transporter protein.
ab128848 staining of dopamine transporter in mouse brain (substantia nigra) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/500) for two hours at room temperature. A Biotin-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
This product has been referenced in:
- Beauvais G et al. Efficient RNA interference-based knockdown of mutant torsinA reveals reversibility of PERK-eIF2a pathway dysregulation in DYT1 transgenic rats in vivo. Brain Res 1706:24-31 (2019). Read more (PubMed: 30366018) »