Overview

  • Product name

    Anti-Dopamine Transporter antibody [6V-23-23]
    See all Dopamine Transporter primary antibodies
  • Description

    Mouse monoclonal [6V-23-23] to Dopamine Transporter
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Does not react with: Human
  • Immunogen

    Synthetic peptide corresponding to Rat Dopamine Transporter (N terminal).

  • Positive control

    • In western blot, this antibody gave a positive signal in mouse and rat forebrain tissue lysates, and in rat brain striatal lysate.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab128848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
IHC-P 1/500.

Target

  • Function

    Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.
  • Involvement in disease

    Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI) [MIM:613135]. It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs.
  • Sequence similarities

    Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • DA transporter antibody
    • DAT 1 antibody
    • DAT antibody
    • DAT1 antibody
    • Dopamine transporter 1 antibody
    • Dopamine transporter antibody
    • PKDYS antibody
    • SC6A3_HUMAN antibody
    • SLC6A3 antibody
    • Sodium dependent dopamine transporter antibody
    • Sodium-dependent dopamine transporter antibody
    • Solute carrier family 6 (neurotransmitter transporter dopamine), member 3 antibody
    • Solute carrier family 6 (neurotransmitter transporter), member 3 antibody
    • Solute carrier family 6 member 3 antibody
    • Variable number tandem repeat (VNTR) antibody
    see all

Images

  • All lanes : Anti-Dopamine Transporter antibody [6V-23-23] (ab128848) at 5 µg/ml

    Lane 1 : Forebrain (Mouse) Tissue Lysate
    Lane 2 : Forebrain (Rat) Tissue Lysate
    Lane 3 : Cerebellum (Rat) Tissue Lysate
    Lane 4 : Brain (Rat) Striatal Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 69 kDa
    Observed band size: 69 kDa
    Additional bands at: 18 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab128848 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.

    Rat cerebellum (lane 3) was included as a negative control.  This tissue does not express dopamine transporter protein. 

  • ab128848 staining of dopamine transporter in mouse brain (substantia nigra) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/500) for two hours at room temperature. A Biotin-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

References

This product has been referenced in:

  • Beauvais G  et al. Efficient RNA interference-based knockdown of mutant torsinA reveals reversibility of PERK-eIF2a pathway dysregulation in DYT1 transgenic rats in vivo. Brain Res 1706:24-31 (2019). Read more (PubMed: 30366018) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted May 16 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted May 31 2014

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