Anti-Dopamine Transporter antibody [6V-23-23] (ab128848)
Key features and details
- Mouse monoclonal [6V-23-23] to Dopamine Transporter
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG1
Overview
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Product name
Anti-Dopamine Transporter antibody [6V-23-23]
See all Dopamine Transporter primary antibodies -
Description
Mouse monoclonal [6V-23-23] to Dopamine Transporter -
Host species
Mouse -
Specificity
Not suitable for rat IHC-P.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat
Does not react with: Human -
Immunogen
Synthetic peptide corresponding to Rat Dopamine Transporter (N terminal).
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Positive control
- In western blot, this antibody gave a positive signal in mouse and rat forebrain tissue lysates, and in rat brain striatal lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
6V-23-23 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 5 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
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IHC-P | (2) |
1/500.
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Notes |
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WB
Use a concentration of 5 µg/ml. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
IHC-P
1/500. |
Target
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Function
Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals. -
Involvement in disease
Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI) [MIM:613135]. It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs. -
Sequence similarities
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 13162 Mouse
- Entrez Gene: 24898 Rat
- SwissProt: Q61327 Mouse
- SwissProt: P23977 Rat
- Unigene: 41993 Mouse
- Unigene: 10093 Rat
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Alternative names
- DA transporter antibody
- DAT 1 antibody
- DAT antibody
see all
Images
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IHC image of Dopamine Transporter staining in a section of formalin-fixed paraffin-embedded mouse brain performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab128848, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Anti-Dopamine Transporter antibody ab128848 was used with Tissue clearing kit – CUBIC (ab316246) and 3D Tissue Staining Kit – CUBIC (ab316248) to penetrate, stain and clear a whole mouse brain. White: nuclear staining, Purple: DAT.
Learn more about tissue clearing kits, reagents, and protocols designed to make it easier to stain whole brains and get more data from each valuable tissue sample.
For a whole mouse brain, we recommend starting with 5 ug of ab128848 and using a Fab fragment secondary antibody with 3.35 µg to create an antibody complex before 3D staining (see protocol for details). Additive A was used during the staining process.
The sample was imaged using a light-sheet microscopy
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All lanes : Anti-Dopamine Transporter antibody [6V-23-23] (ab128848) at 5 µg/ml
Lane 1 : Forebrain (Mouse) Tissue Lysate
Lane 2 : Forebrain (Rat) Tissue Lysate
Lane 3 : Cerebellum (Rat) Tissue Lysate
Lane 4 : Brain (Rat) Striatal Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Additional bands at: 18 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab128848 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.
Rat cerebellum (lane 3) was included as a negative control. This tissue does not express dopamine transporter protein.
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ab128848 staining of dopamine transporter in mouse brain (substantia nigra) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat-mediated antigen retrieval was carried out using citric acid. Samples were incubated with primary antibody (1/500) for two hours at room temperature. A Biotin-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab128848 has been referenced in 4 publications.
- Morin A et al. Global Cerebral Ischemia in Male Long Evans Rats Impairs Dopaminergic/ΔFosB Signalling in the Mesocorticolimbic Pathway Without Altering Delay Discounting Rates. Front Behav Neurosci 15:770374 (2021). PubMed: 35058756
- Beauvais G et al. Efficient RNA interference-based knockdown of mutant torsinA reveals reversibility of PERK-eIF2a pathway dysregulation in DYT1 transgenic rats in vivo. Brain Res 1706:24-31 (2019). PubMed: 30366018
- Wang G et al. Age-Associated Dopaminergic Neuron Loss and Midbrain Glia Cell Phenotypic Polarization. Neuroscience 415:89-96 (2019). PubMed: 31325563
- Hansson J et al. Overexpression of Functional SLC6A3 in Clear Cell Renal Cell Carcinoma. Clin Cancer Res 23:2105-2115 (2017). PubMed: 27663598