Key features and details
- Goat polyclonal to Dopamine Transporter
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Dopamine Transporter antibody
See all Dopamine Transporter primary antibodies
DescriptionGoat polyclonal to Dopamine Transporter
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Orangutan
- Human Brain (Substantia nigra) lysate
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab130140 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab130140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 68 kDa).
1 hour primary incubation is recommended for this product.
FunctionAmine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.
Involvement in diseaseDefects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI) [MIM:613135]. It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs.
Sequence similaritiesBelongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.
- Information by UniProt
- DA transporter antibody
- DAT 1 antibody
- DAT antibody
Anti-Dopamine Transporter antibody (ab130140) at 1 µg/ml + Human Substantia nigra lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Mazei-Robison et al, J Neurosci. 2008 Jul 9;28(28):7040-6. PMID: 18614672).
ab130140 has not yet been referenced specifically in any publications.