Key features and details
- Rabbit polyclonal to Dopamine Transporter
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-Dopamine Transporter antibody
See all Dopamine Transporter primary antibodies
DescriptionRabbit polyclonal to Dopamine Transporter
Tested applicationsSuitable for: Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Human
- Rat kidney tissue; RSC96 cells
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab214470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/20 - 1/100.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes|
FunctionAmine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.
Involvement in diseaseDefects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI) [MIM:613135]. It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs.
Sequence similaritiesBelongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.
- Information by UniProt
- DA transporter antibody
- DAT 1 antibody
- DAT antibody
Flow Cytometric analysis of RSC96 cells labeling Dopamine Transporter using ab214470 at 6 µg for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (green) compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Immunohistochemical analysis of formalin-fixed paraffin-embedded rat kidney tissue labeling Dopamine Transporter with ab214470 at 1/400 dilution, followed by conjugation to the secondary antibody and DAB staining.
ab214470 has not yet been referenced specifically in any publications.