Anti-Dopamine Transporter antibody (ab214470)
Key features and details
- Rabbit polyclonal to Dopamine Transporter
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Dopamine Transporter antibody
See all Dopamine Transporter primary antibodies -
Description
Rabbit polyclonal to Dopamine Transporter -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human -
Immunogen
Synthetic peptide within Human Dopamine Transporter aa 550 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q01959 -
Positive control
- Rat kidney tissue; RSC96 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/20 - 1/100.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes
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Notes |
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Flow Cyt
1/20 - 1/100. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes |
Target
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Function
Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals. -
Involvement in disease
Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI) [MIM:613135]. It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs. -
Sequence similarities
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6531 Human
- Entrez Gene: 13162 Mouse
- Entrez Gene: 24898 Rat
- Omim: 126455 Human
- SwissProt: Q01959 Human
- SwissProt: Q61327 Mouse
- SwissProt: P23977 Rat
- Unigene: 406 Human
see all -
Alternative names
- DA transporter antibody
- DAT 1 antibody
- DAT antibody
see all
Images
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Flow Cytometric analysis of RSC96 cells labeling Dopamine Transporter using ab214470 at 6 µg for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (green) compared to control cells (blue), secondary only (light blue) and isotype control (orange).
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Immunohistochemical analysis of formalin-fixed paraffin-embedded rat kidney tissue labeling Dopamine Transporter with ab214470 at 1/400 dilution, followed by conjugation to the secondary antibody and DAB staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab214470 has not yet been referenced specifically in any publications.