Overview

  • Product name
    Anti-Doppel antibody
  • Description
    Rabbit polyclonal to Doppel
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    TQAANQGEF

    , corresponding to amino acids 112-120 of Human Doppel.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Mouse spleen 100k x g supernatant for Western blot.

Properties

Applications

Our Abpromise guarantee covers the use of ab23701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 20 kDa.
IHC-P Use a concentration of 1 µg/ml.

Target

Images

  • Western blot - Anti-Doppel antibody (ab23701)
    Western blot - Anti-Doppel antibody (ab23701)
    All lanes : Anti-Doppel antibody (ab23701) at 2 µg/ml

    Lane 1 : 27ug mouse spleen 100k x g supernatant.
    Lane 2 : 54ug mouse spleen 100k x g supernatant.

    Performed under reducing conditions.

    Predicted band size: 20 kDa
    Observed band size: 19 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 25 kDa (possible glycosylated form)

References

ab23701 has not yet been referenced specifically in any publications.

Publishing research using ab23701? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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2 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-Doppel antibody

 Poor
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (CNS)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
CNS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Mar 24 2017

Western blot abreview for Anti-Doppel antibody

 Poor
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (CNS)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
CNS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted Nov 18 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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