Anti-Doppel antibody (ab23701)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-Doppel antibody
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DescriptionRabbit polyclonal to Doppel
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide:
TQAANQGEF
, corresponding to amino acids 112-120 of Human Doppel. -
Positive control
- Mouse spleen 100k x g supernatant for Western blot.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 0.1% BSA, Tris buffered saline, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab23701 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 2 µg/ml. Predicted molecular weight: 20 kDa. | |
| IHC-P | Use a concentration of 1 µg/ml. |
Target
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Tissue specificityExpressed in testis.
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Sequence similaritiesBelongs to the prion family.
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Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 23627 Human
- Entrez Gene: 26434 Mouse
- Entrez Gene: 113910 Rat
- Omim: 604263 Human
- SwissProt: Q9UKY0 Human
- SwissProt: Q9QUG3 Mouse
- Unigene: 406696 Human
- Unigene: 180750 Mouse
see all -
Alternative names
- dJ1068H6.4 antibody
- DPL antibody
- Dublet antibody
see all
Images
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Western blot - Anti-Doppel antibody (ab23701)All lanes : Anti-Doppel antibody (ab23701) at 2 µg/ml
Lane 1 : 27ug mouse spleen 100k x g supernatant.
Lane 2 : 54ug mouse spleen 100k x g supernatant.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa (possible glycosylated form)
Datasheets and documents
References
ab23701 has not yet been referenced specifically in any publications.
