• Product name
    Anti-Doublecortin antibody [2G5]
    See all Doublecortin primary antibodies
  • Description
    Mouse monoclonal [2G5] to Doublecortin
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 362-411 of Human Doublecortin expressed in E. Coli.

  • Positive control
    • Human Doublecortin recombinant protein; Mouse heart lysate; HepG2 and SK-N-SH cells; Human brain and kidney cancer tissues.



Our Abpromise guarantee covers the use of ab135349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 49 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


ICC/IF 1/200 - 1/1000.


  • Function
    Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.
  • Tissue specificity
    Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
  • Involvement in disease
    Defects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'.
    Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal.
    Note=A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1).
  • Sequence similarities
    Contains 2 doublecortin domains.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • DBCN antibody
    • Dbct antibody
    • DC antibody
    • DCX antibody
    • DCX_HUMAN antibody
    • Doublecortex antibody
    • Doublin antibody
    • FLJ51296 antibody
    • Lis X antibody
    • Lis-X antibody
    • Lissencephalin X antibody
    • Lissencephalin-X antibody
    • Lissencephaly X linked antibody
    • Lissencephaly X linked doublecortin antibody
    • LISX antibody
    • Neuronal migration protein doublecortin antibody
    • OTTHUMP00000023859 antibody
    • OTTHUMP00000023860 antibody
    • OTTHUMP00000216315 antibody
    • OTTHUMP00000216316 antibody
    • SCLH antibody
    • XLIS antibody
    see all


  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling Doublecortin with ab135349 at 1/200 dilution followed by DAB staining.
  • Flow cytometric analysis of SK-N-SH cells labelling Doublecortin with ab135349 at 1/200 dilution (green) or negative control (red).
  • Immunofluorescent analysis of HepG2 cells labelling Doublecortin with ab135349 at 1/200 dilution (green). DRAQ5 fluorescent DNA dye (blue).
  • Immunohistochemical analysis of paraffin-embedded Human kidney cancer tissue labelling Doublecortin with ab135349 at 1/200 dilution followed by DAB staining.
  • Anti-Doublecortin antibody [2G5] (ab135349) at 1/500 dilution + Mouse heart lysate

    Predicted band size: 49 kDa

  • Anti-Doublecortin antibody [2G5] (ab135349) at 1/500 dilution + Recombinant Human Doublecortin protein

    Predicted band size: 49 kDa

    Expected MW of recombinant protein is 34.1 kDa.


This product has been referenced in:
  • Kreiner G  et al. Lack of riluzole efficacy in the progression of the neurodegenerative phenotype in a new conditional mouse model of striatal degeneration. PeerJ 5:e3240 (2017). IHC-P ; Mouse . Read more (PubMed: 28462043) »
  • Fan WJ  et al. Neural differentiation and synaptogenesis in retinal development. Neural Regen Res 11:312-8 (2016). IF . Read more (PubMed: 27073386) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Mouse Tissue sections (Brain)
Blocking step
Normal goat serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Dec 22 2015

Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Mouse Tissue sections (Cortical)
Yes - 0.3% Triton X

Abcam user community

Verified customer

Submitted Jun 24 2013


I received an anti-DCX antibody (ab135349 a few weeks ago. I have tried a variety of of fixes and tissues, but the antibody does not work. Detailed below are the various techniques I've tried:

1. Multiple rounds of fresh-frozen cryofixed E14 brains, with sections fixed in 4% PFA or FAA for 10-20 minutes. Antigen-retrieved with and without boiling Sodium Citrate (pH 6) for 10-20 minutes. 1 hour blocking in 5% secondary-specific blocking solution with Triton, 1 hour room temperature or overnight at 4 degrees, washed 3x 5 min with PBS, 1 hour secondary (multiple ones), washed 3x 5 min PBS and coverslipped.

2. Multiple PFA-fixed E14-16 brains (heads overnight in PFA), cryofixed, cut, and stained according to above protocol.

3. FAA fixed paraffinized E16 and P30 brains, deparaffinized and antigen-retrieved with boiling sodium citrate. Staining protocol followed as above.

I've attached a few pictures of my most recent staining attempt (fresh-frozen, PFA and FAA fixed) along with staining done with the exact same secondary for a different primary antibody (same experiment, secondary control). I've also attached a picture of previous staining with your Rabbit DCX antibody at both E14 cortex and P60 hippocampus.

Could I please exchange my non-working ms anti-DCX for a rb anti-DCX (catalog #ab18723)? I should have known better than to order a different antibody than the one I know works. I've gotten great staining with your rabbit anti-DCX and would just rather have that one than try to QC another ms anti-DCX.

Read More

Thank you for taking time to contact us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

I apologize the the attachments didn't go through. Was the trouble that you weren't seeing any staining (or high background, etc.)? Could you try sending the images againas a .doc, .ppt, .pdf, or .jpg format?

What dilution of the antibody had you tried?

I would be happy to send youa replacement of the rabbit polyclonal, ab18723, but would you be able to confirm the above? I just want to make sure we have as much information about this for our records.

I apologize for the inconvenienceand I appreciateyour cooperation. I look forward to hearing from you.

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