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Our Abpromise guarantee covers the use of ab113435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa).|
|IHC-P||Use a concentration of 2 - 4 µg/ml.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebellum labeling Doublecortin with ab113435 at 2µg/ml. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"