Anti-Doublecortin antibody (ab153668)
Key features and details
- Chicken polyclonal to Doublecortin
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Drosophila melanogaster
- Isotype: IgY
Overview
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Product name
Anti-Doublecortin antibody
See all Doublecortin primary antibodies -
Description
Chicken polyclonal to Doublecortin -
Host species
Chicken -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Mouse
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Immunogen
Two KLH conjugated synthetic peptides corresponding to different regions within Human Doublecortin (CAA06617.1) and mouse Doublecortin (AAT58219.1).
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General notes
Do not freeze this antibody unless you want to store them for longer periods of time.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Mouse
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| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/1000 - 1/2000.
Use 2% paraformaldehyde-fixed cells. |
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| IHC-P |
1/1000.
4% PFA-fixed, paraffin-embedded section. |
| Notes |
|---|
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ICC/IF
1/1000 - 1/2000. Use 2% paraformaldehyde-fixed cells. |
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IHC-P
1/1000. 4% PFA-fixed, paraffin-embedded section. |
Target
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Function
Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration. -
Tissue specificity
Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas. -
Involvement in disease
Defects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'.
Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal.
Note=A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1). -
Sequence similarities
Contains 2 doublecortin domains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 13193 Mouse
- SwissProt: O88809 Mouse
- Unigene: 12871 Mouse
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Alternative names
- DBCN antibody
- Dbct antibody
- DC antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody (ab153668)
Immunohistochemistry (4% PFA-fixed, paraffin-embedded) analysis of e18 mouse cochlear ganglion tissue labelling Doublecortin (green) with ab153668. Tissue was fixed with 4% paraformaldehyde. Red - rabbit anti-Beta-Tubulin 3.
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Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody (ab153668)Immunocytochemistry/Immunofluorescence analysis of SpH64 cells in culture labelling Doublecortin (green) with ab153668. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-Golgi Marker.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody (ab153668)Immunohistochemistry (4% PFA-fixed, paraffin-embedded section) analysis of e18 mouse brain (periventricular zone) tissue labelling Doublecortin (red) with ab153668. Green - GFP autofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab153668 has been referenced in 3 publications.
- Kuo YY et al. Endonuclease VIII-like 1 deficiency impairs survival of newly generated hippocampal neurons and memory performance in young-adult male mice. Life Sci 254:117755 (2020). PubMed: 32437792
- Will L et al. Dynein activating adaptor BICD2 controls radial migration of upper-layer cortical neurons in vivo. Acta Neuropathol Commun 7:162 (2019). PubMed: 31655624
- Ghuman H et al. Long-term retention of ECM hydrogel after implantation into a sub-acute stroke cavity reduces lesion volume. Acta Biomater 63:50-63 (2017). PubMed: 28917705