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    doublecortin-antibody-ab153668.pdf

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Neuroscience Cell Adhesion Proteins Cytoskeletal Proteins Microtubules
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Anti-Doublecortin antibody (ab153668)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody (ab153668)
  • Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody (ab153668)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody (ab153668)

Key features and details

  • Chicken polyclonal to Doublecortin
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Mouse, Drosophila melanogaster
  • Isotype: IgY

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Overview

  • Product name

    Anti-Doublecortin antibody
    See all Doublecortin primary antibodies
  • Description

    Chicken polyclonal to Doublecortin
  • Host species

    Chicken
  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    See all applications and species data
  • Immunogen

    Two KLH conjugated synthetic peptides corresponding to different regions within Human Doublecortin (CAA06617.1) and mouse Doublecortin (AAT58219.1).

  • General notes

    Do not freeze this antibody unless you want to store them for longer periods of time.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    pH: 7
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Microtubules
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Growth Cone
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Neuronal

Associated products

  • Isotype control

    • Chicken IgY - Isotype Control (ab50579)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab153668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Mouse
Application Abreviews Notes
ICC/IF
1/1000 - 1/2000.

Use 2% paraformaldehyde-fixed cells.

IHC-P
1/1000.

4% PFA-fixed, paraffin-embedded section.

Notes
ICC/IF
1/1000 - 1/2000.

Use 2% paraformaldehyde-fixed cells.

IHC-P
1/1000.

4% PFA-fixed, paraffin-embedded section.

Target

  • Function

    Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.
  • Tissue specificity

    Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
  • Involvement in disease

    Defects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'.
    Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal.
    Note=A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1).
  • Sequence similarities

    Contains 2 doublecortin domains.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession O43602 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 13193 Mouse
    • SwissProt: O88809 Mouse
    • Unigene: 12871 Mouse
    • Alternative names

      • DBCN antibody
      • Dbct antibody
      • DC antibody
      • DCX antibody
      • DCX_HUMAN antibody
      • Doublecortex antibody
      • Doublin antibody
      • FLJ51296 antibody
      • Lis X antibody
      • Lis-X antibody
      • Lissencephalin X antibody
      • Lissencephalin-X antibody
      • Lissencephaly X linked antibody
      • Lissencephaly X linked doublecortin antibody
      • LISX antibody
      • Neuronal migration protein doublecortin antibody
      • OTTHUMP00000023859 antibody
      • OTTHUMP00000023860 antibody
      • OTTHUMP00000216315 antibody
      • OTTHUMP00000216316 antibody
      • SCLH antibody
      • XLIS antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody (ab153668)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody (ab153668)

      Immunohistochemistry (4% PFA-fixed, paraffin-embedded) analysis of e18 mouse cochlear ganglion tissue labelling Doublecortin (green) with ab153668. Tissue was fixed with 4% paraformaldehyde. Red - rabbit anti-Beta-Tubulin 3.

    • Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody (ab153668)
      Immunocytochemistry/ Immunofluorescence - Anti-Doublecortin antibody (ab153668)

      Immunocytochemistry/Immunofluorescence analysis of SpH64 cells in culture labelling Doublecortin (green) with ab153668. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-Golgi Marker.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody (ab153668)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Doublecortin antibody (ab153668)

      Immunohistochemistry (4% PFA-fixed, paraffin-embedded section) analysis of e18 mouse brain (periventricular zone) tissue labelling Doublecortin (red) with ab153668. Green - GFP autofluorescence.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab153668? Please let us know so that we can cite the reference in this datasheet.

    ab153668 has been referenced in 3 publications.

    • Kuo YY  et al. Endonuclease VIII-like 1 deficiency impairs survival of newly generated hippocampal neurons and memory performance in young-adult male mice. Life Sci 254:117755 (2020). PubMed: 32437792
    • Will L  et al. Dynein activating adaptor BICD2 controls radial migration of upper-layer cortical neurons in vivo. Acta Neuropathol Commun 7:162 (2019). PubMed: 31655624
    • Ghuman H  et al. Long-term retention of ECM hydrogel after implantation into a sub-acute stroke cavity reduces lesion volume. Acta Biomater 63:50-63 (2017). PubMed: 28917705

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