Question (22246) | Anti-Doublecortin antibody (ab18723)

Go to datasheet (ab18723)

Question

Our customer advances an enquiry about the ab18723. This customer would like to conduct the IHC and ICC of mouse sample. But the host of ab18723 is also mouse. In order to decrease the background, is there any solution to resolve this problem? Would you please help this customer deal with this issue?

Answer

Thank you for contacting us with your question. Although we generally don't recommend using a primary antibody raised in mouse to stain mouse tissue, there are steps that can reduce the background when this kind of staining is necessary. For mouse on mouse staining, the endogenous IgG molecules in the tissue should be blocked by anti-mouse IgG Fab fragments, such as ab6668. The protocol would be as follows: 1) Prepare tissue or cell samples as usual and perform the usual protein block (10% non-mouse serum for 1 hour). 2) Wash 3x2 minutes in TBS and 0.025% Triton x-100 with gentle agitation. 3) Incubate sections with an unconjugated anti-mouse IgG Fab fragment for 1 hour at room temperature or overnight at 4C. 4) Proceed with antibody staining. I hope this information helps, but please let me know if your customer has any further questions.

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