Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Doublecortin antibody - Neuronal Marker

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain)
Specification
brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH9.0, 20 min, 100C
Permeabilization
No

Other product details

Dilution
1/8000
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: Dako diluent

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit anti-mouse IgG, Leica Refine
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer

Additional data

Additional Notes
Multiple antigen retrieval regimes employed EDTA gives best signal/background results

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Submitted Oct 11 2011

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