Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Doublecortin antibody

Poor
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Hippocampal tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
Yes - TBST
Specification
Hippocampal tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s)
Dilution
1/200

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Perkin Elmer Opal secondary
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Abcam response
Thank you for your valuable feedback. ab18723 has not been previously tested in dog sample. It is possible that this antibody may not be suitable for use in this species. We welcome other Abreviews to determine this.

Dr. Tom Duncan

Verified customer

Submitted Jan 05 2018

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