Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Doublecortin antibody

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Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Common marmoset Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - Triton X100 0.2%
Specification
Brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde

Other product details

Incubation time
48 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Phosphate buffer 0.1M
Dilution
1/1000

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: goat anti rabbit AlexaFl
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 488

Additional data

Additional Notes
Floating 30 ´m sections of marmoset brain (4% PFA fixed, frozen sections) Blocking solution : PB 0.1M with 0.1% Tween and 3% serum Primary antibody was diluted in the blocking solution Nuclei were stained with DAPI Section shown: dentate gyrus of the hippocampus

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Submitted Dec 13 2017

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