Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Doublecortin antibody

Excellent
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Spinal Cord)
Antigen retrieval step
None
Permeabilization
Yes - Triton X100
Specification
Spinal Cord
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking solution
Dilution
1/200

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Cy™3 AffiniPure Goat Anti-Rabbit IgG (H+L) (min X
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®

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Submitted Aug 22 2018

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