Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Doublecortin antibody - Neuronal Marker

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain svr: progenitor olfactory neurones)
Specification
brain svr: progenitor olfactory neurones
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1

Other product details

Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used:
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594

Additional data

Additional Notes
The MIP image was derived from Apotome-generated Z-stacks from the greyscale image of each of the channels in the MIP.

Abcam DCX at 1/2000 (Alexa 594- red)
Non-Abcam mouse anti Ki67 1/1000 (Alexa 488- green)
Hoechst- blue.

Both antibodies were incubated overnight at RT (Hoechst 2 hours at RT).

Mr. Carl Hobbs

Verified customer

Submitted Feb 21 2007

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