Western blot abreview for Anti-Doublecortin antibody

Excellent
Abreviews
Application
Western blot
Sample
Agelaius phoeniceus (Red-winged blackbird) Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (10% AA-BIS gel)
Loading amount
10 µg
Specification
Brain
Blocking step
Fish gelatin in TBS as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 0.5% · Temperature: 22°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 0.05% fish gelatin in TBS-T
Dilution
1/1000

Secondary antibody

Dilution
1/20000
Name
Non-Abcam antibody was used: IRDye® 800CW Goat anti-Rabbit IgG (H + L)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Fluorescence
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 45 kDa Non-specific: 25, 75 kDa

Prof. Argel Aguilar Valles

Verified customer

Submitted Apr 02 2019

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