Overview

  • Product name
    Anti-Doublecortin antibody
    See all Doublecortin primary antibodies
  • Description
    Goat polyclonal to Doublecortin
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control
    • ICC/IF: Post-natal rat pup (PND1) heterogeneous brain cells. WB: Post-natal rat pup whole brain lysate (PND2-6)* and rat pup PND2-6 – minimal cortex brain lysate. Doublecortin (human) overexpressing HEK-293T lysate C-Myc/DDK tagged. IHC-P: Mouse dentate gyrus tissue. Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab235153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 49 kDa.
ICC/IF Use a concentration of 15 µg/ml.

Target

  • Function
    Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.
  • Tissue specificity
    Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
  • Involvement in disease
    Defects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'.
    Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal.
    Note=A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1).
  • Sequence similarities
    Contains 2 doublecortin domains.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DBCN antibody
    • Dbct antibody
    • DC antibody
    • DCX antibody
    • DCX_HUMAN antibody
    • Doublecortex antibody
    • Doublin antibody
    • FLJ51296 antibody
    • Lis X antibody
    • Lis-X antibody
    • Lissencephalin X antibody
    • Lissencephalin-X antibody
    • Lissencephaly X linked antibody
    • Lissencephaly X linked doublecortin antibody
    • LISX antibody
    • Neuronal migration protein doublecortin antibody
    • OTTHUMP00000023859 antibody
    • OTTHUMP00000023860 antibody
    • OTTHUMP00000216315 antibody
    • OTTHUMP00000216316 antibody
    • SCLH antibody
    • XLIS antibody
    see all

Images

  • 4% PFA fixed, 0.3% Triton X-100 permeabilized post-natal rat pup (PND1) heterogeneous brain cells labeling Doublecortin using ab235153 at 15 µg/ml (O/N at 4°C) (green) in ICC/IF.The Secondary antibody is a Donkey anti-Gt IgG DyLigh™ 488 conjugated preadsorbed antibody, used at 5 µg/ml for 1 hour at room temperature. The Nuclear counterstain is DAPI (Blue).

  • All lanes : Anti-Doublecortin antibody (ab235153) at 1/1000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 15 µg
    Lane 2 : Post-natal rat pup whole brain lysate (PND2-6)* at 15 µg
    Lane 3 : Rat pup PND2-6 - minimal cortex brain lysate at 15 µg
    Lane 4 : Rat pup PND2-6 – minimal cortex brain lysate at 35 µg

    Secondary
    Lanes 1-2 & 4 : Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125) at 1/40000 dilution


    Predicted band size: 49 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    * "Doublecortin microtubule affinity is regulated by a balance of kinase and phosphatase activity at the leading edge of migrating neurons." Schaar B.T., Kinoshita K., McConnell S.K. Neuron 41:203213 (2004).

    Minimal cortex defined: small portion of cortex removed prior to lysis.

  • Formalin-fixed, paraffin-embedded mouse dentate gyrus tissue stained for Doublecortin with ab235153 at a 1/200 dilution (30 mins at room temperature) in immunohistochemical analysis. A Donkey anti Goat (HRP) secondary was used at 4 µl/ml (20 mins at room temperature). Counterstained with hematoxylin. HIER using citrate buffer for 20 mins.

    Pathologist analysis: Doublecortin (DCX) showed selective staining of soma and nuclei of cells in the dentate gyrus in mouse brain.  The specificity of the staining is consistent with DCX staining in the Human Protein Atlas and as such, is suitable for IHC at a concentration of 1/200.

  • 4% PFA fixed, 0.3% Triton X-100 permeabilized post-natal rat pup (PND1) heterogeneous brain cells labeling Doublecortin using ab235153 at 15 µg/ml (O/N at 4°C) (green) in ICC/IF. The Secondary antibody is a Donkey anti-Gt IgG DyLigh™ 488 conjugated preadsorbed, used at 5 µg/ml for 1 hour at room temperature. The Nuclear counterstain is DAPI (Blue).

  • All lanes : Anti-Doublecortin antibody (ab235153) at 1/1000 dilution

    Lane 1 : Doublecortin (human) overexpressing HEK-293T lysate C-Myc/DDK tagged
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 3 : Adult mouse normal brain tissue lysate
    Lane 4 : Adult rat normal brain tissue lysate

    Secondary
    All lanes : Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125) at 1/40000 dilution

    Predicted band size: 49 kDa
    Observed band size: 48, 35 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds
  • Human brain tissue stained for Doublecortin using ab235153 at 1/1000 dilution in immunohistochemical analysis. The counter stain is hematoxylin. 

    Heat induced epitope retrieval (HIER).

     

References

ab235153 has not yet been referenced specifically in any publications.

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