Overview

  • Product name
    Anti-Doublecortin antibody
    See all Doublecortin primary antibodies
  • Description
    Goat polyclonal to Doublecortin
  • Host species
    Goat
  • Tested applications
    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Doublecortin aa 69-81 (internal sequence).
    Sequence:

    DRSHCQSLRFHQN


    Database link: NP_000546.2

  • Positive control
    • ICC/IF: HepG2 and NIH/3T3 cells. Flow: Kelly cells

Properties

Applications

Our Abpromise guarantee covers the use of ab238253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.
  • Tissue specificity
    Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
  • Involvement in disease
    Defects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'.
    Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal.
    Note=A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1).
  • Sequence similarities
    Contains 2 doublecortin domains.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DBCN antibody
    • Dbct antibody
    • DC antibody
    • DCX antibody
    • DCX_HUMAN antibody
    • Doublecortex antibody
    • Doublin antibody
    • FLJ51296 antibody
    • Lis X antibody
    • Lis-X antibody
    • Lissencephalin X antibody
    • Lissencephalin-X antibody
    • Lissencephaly X linked antibody
    • Lissencephaly X linked doublecortin antibody
    • LISX antibody
    • Neuronal migration protein doublecortin antibody
    • OTTHUMP00000023859 antibody
    • OTTHUMP00000023860 antibody
    • OTTHUMP00000216315 antibody
    • OTTHUMP00000216316 antibody
    • SCLH antibody
    • XLIS antibody
    see all

Images

  • Paraformaldehyde-fixed, 0.15% triton permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Doublecortin (green) using ab238253 at 10 μg/mL in ICC/IF, followed by Alexa Fluor 488 secondary antibody (2μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue).
    Negative control: Unimmunized goat IgG (10μg/ml) followed by Alexa Fluor 488 secondary antibody (2μg/ml).

  • Paraformaldehyde-fixed, 0.15% triton permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells stained for Doublecortin (green) using ab238253 at 10 μg/mL in ICC/IF, followed by Alexa Fluor 488 secondary antibody (2μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue).
    Negative control: Unimmunized goat IgG (10μg/ml) followed by Alexa Fluor 488 secondary antibody (2μg/ml).

  • Flow cytometric analysis of paraformaldehyde-fixed, 0.5% triton permeabilzed Kelly cells labeling Doublecortin using ab238253 at 10 μg/mL (Blue) followed by Alexa Fluor 488 secondary antibody 1 μg/mL.
    IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

References

ab238253 has not yet been referenced specifically in any publications.

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