Key features and details
- Goat polyclonal to Doublecortin
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Doublecortin antibody
See all Doublecortin primary antibodies
DescriptionGoat polyclonal to Doublecortin
Tested applicationsSuitable for: Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
- ICC/IF: HepG2. Flow: Kelly cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab238253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 10 µg/ml.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionSeems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.
Tissue specificityHighly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
Involvement in diseaseDefects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'.
Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal.
Note=A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1).
Sequence similaritiesContains 2 doublecortin domains.
- Information by UniProt
- DBCN antibody
- Dbct antibody
- DC antibody
Paraformaldehyde-fixed, 0.15% triton permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Doublecortin (green) using ab238253 at 10 μg/mL in ICC/IF, followed by Alexa Fluor 488 secondary antibody (2μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10μg/ml) followed by Alexa Fluor 488 secondary antibody (2μg/ml).
Flow cytometric analysis of paraformaldehyde-fixed, 0.5% triton permeabilzed Kelly (human cell line) cells labeling Doublecortin using ab238253 at 10 μg/mL (Blue) followed by Alexa Fluor 488 secondary antibody 1 μg/mL.
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238253 has not yet been referenced specifically in any publications.