Overview

  • Product name
    Anti-Doublecortin antibody [EPR10935(B)]
    See all Doublecortin primary antibodies
  • Description
    Rabbit monoclonal [EPR10935(B)] to Doublecortin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Doublecortin aa 100-200. The exact sequence is proprietary. Synthetic peptide, corresponding to residues in Human Doublecortin (UniProt: O43602).
    Database link: O43602

  • Positive control
    • Human fetal brain and C6 lysates; C6 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab167400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 49 kDa.
Flow Cyt 1/100 - 1/500.

 

 

 

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.
    • Tissue specificity
      Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
    • Involvement in disease
      Defects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'.
      Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal.
      Note=A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1).
    • Sequence similarities
      Contains 2 doublecortin domains.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • DBCN antibody
      • Dbct antibody
      • DC antibody
      • DCX antibody
      • DCX_HUMAN antibody
      • Doublecortex antibody
      • Doublin antibody
      • FLJ51296 antibody
      • Lis X antibody
      • Lis-X antibody
      • Lissencephalin X antibody
      • Lissencephalin-X antibody
      • Lissencephaly X linked antibody
      • Lissencephaly X linked doublecortin antibody
      • LISX antibody
      • Neuronal migration protein doublecortin antibody
      • OTTHUMP00000023859 antibody
      • OTTHUMP00000023860 antibody
      • OTTHUMP00000216315 antibody
      • OTTHUMP00000216316 antibody
      • SCLH antibody
      • XLIS antibody
      see all

    Images

    • All lanes : Anti-Doublecortin antibody [EPR10935(B)] (ab167400) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : C6 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 49 kDa

    • Flow cytometric analysis of permeabilized C6 cells labeling Doublecortin with ab167400 at 1/100 dilution (red) or a Rabbit IgG (negative)(green).

    References

    ab167400 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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