Overview

  • Product name
    DoubleStain CL IHC Kit: G&Rt on human/mouse tissue (HRP/Green & AP/Red)
  • Assay type
    Polymer Multiplex
  • Product overview

    Abcam's Doublestain IHC Kit (ab183282) is designed to be used with user supplied goat and rat primary antibodies to detect two distinct antigens on human or mouse tissue or cell samples. The kit has been tested on paraffin–embedded human and mouse tissues.

     

    Double staining is one of most common methods used in immunohistostaining allowing for the detection of two distinct antigens in a single tissue. Abcam's Doublestain IHC Kit (ab183282) supplies the user with two polymer enzyme conjugates: Goat AP Polymer and Rat HRP Polymer with two distinct substrates/chromogens, Emerald and Permanent Red. With this protocol the goat and rat primary antibodies will be applied to the tissue at the same time but the user will sequentially apply the polymer enzyme conjugate to the specimen. When two proteins are present in the same location a blue or purple color will develop depending on the quantity of each antigen screened. If only the anti-goat antigen is present only the Permanent Red chromogen will be visible and if only the Rat antigen is present then only the Emerald chromogen will be seen. Abcam's Doublestain IHC Kit (ab183282) is a non-biotin system avoiding endogenous biotin non-specific binding.

  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 12 ml 36 ml 120 ml
    DoubleStain Blocker 1 x 6ml 1 x 18ml 1 x 60ml
    Emerald Chromogen 1 x 7ml 1 x 18ml 1 x 70ml
    Goat AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Non-aqueous Mounting Medium 1 x 12ml 1 x 12ml 2 x 12ml
    Permanent Red Activator (5x) 1 x 3ml 1 x 7.2ml 1 x 14ml
    Permanent Red Chromogen (100x) 1 x 150µl 1 x 360µl 1 x 0.7ml
    Permanent Red Substrate 1 x 15ml 2 x 18ml 1 x 70ml
    Rat HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Rat Primer 1 x 6ml 1 x 18ml 1 x 60ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Protocols

References

ab183282 has not yet been referenced specifically in any publications.

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