Product nameDoubleStain Co-Localization IHC Kit: M&M on rodent tissue (Green/HRP & AP/Red)
Assay typePolymer Multiplex
Abcam's Doublestain IHC Kit (ab183275) is designed to use with two user supplied mouse antibodies to detect two distinct antigens on mouse and rat tissue or cell samples. The advantage of this kit series is that it will allow you to visualize when two proteins are co localized by producing a third color blue purple. Specimens can be frozen or paraffin embedded, or freshly prepared monolayer cell smears.
Double staining is a common method used in immunohistochemistry that allows for detection of two distinct antigens in a single tissue. This kit uses an HRP or AP polymer based technology combined with a proprietary blocking buffer system that achieves ultra sensitivity with no background or cross reactivity. Abcam's Doublestain IHC Kit (ab183275) supplies the user with primer system to enhance the two polymer enzyme conjugates; Mouse HRP Polymer and Mouse AP Polymer with two distinct substrates/chromogens, Permanent Red and Emerald. Permanent Red reacts with Mouse AP Polymer conjugate to produce a red color. Emerald chromogen reacts with Mouse HRP Polymer conjugate to produce a green color. However when the chromogens are produced in the same place the color appears blue to purple in color. Abcam's Doublestain IHC Kit (ab183275) is a non-biotin system that avoids the extra steps involved in blocking non-specific binding due to endogenous biotin.
Tested applicationsSuitable for: IHC-P, IHC-Frmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 12 ml 36 ml 120 ml — Antibody Blocker (40x) 2 x 15ml 1 x 50ml 1 x 125ml Blocker A 1 x 6ml 1 x 18ml 1 x 60ml Blocker B 1 x 6ml 1 x 18ml 1 x 60ml Emerald Chromogen 1 x 7ml 1 x 18ml 1 x 70ml Mouse AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Mouse HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Mouse Primer 1 x 6ml 1 x 18ml 1 x 60ml Non-aqueous Mounting Medium 1 x 6ml 2 x 9ml Permanent Red Activator (5x) 1 x 1.4ml 1 x 3.6ml 1 x 12ml Permanent Red Chromogen (100x) 1 x 70µl 1 x 180µl 1 x 0.6ml Permanent Red Substrate 1 x 7ml 1 x 18ml 1 x 60ml Plastic pipette 1 unit 1 unit 1 unit
Our Abpromise guarantee covers the use of ab183275 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
ab183275 has not yet been referenced specifically in any publications.