DoubleStain IHC kit: G&M on human tissue (BCIP & AEC) (ab183271)
Overview
-
Product name
DoubleStain IHC kit: G&M on human tissue (BCIP & AEC) -
Assay type
Polymer Multiplex -
Product overview
The DoubleStain IHC kit (ab183271) is designed to use with user supplied goat and mouse antibodies to detect two distinct antigens on human tissue or cell samples. This kit has been tested in paraffin tissues, however it can also be used on frozen specimens and freshly prepared monolayer cell smears.
Double staining is a common method used in immunohistochemistry for the detection of two distinct antigens in a single tissue. Abcam's DoubleStain IHC Kit supplies two polymer enzyme conjugates: AP polymer anti-Goat IgG and HRP(AEC) polymer anti-Mouse IgG with two distinct substrates/chromogens, BCIP/NBT (Purple) and AEC (Red). Users will apply the two enzyme conjugates onto the specimen sequentially. If only the anti-goat antigen is present only the BCIP/NBT(Purple) will be present and if the mouse antigen is present only the AEC(Red) will be present. Abcam's DoubleStain IHC Kit is a non-biotin system avoiding endogenous biotin non-specific binding.
-
Tested applications
Suitable for: IHC-Fr, IHC-Pmore details
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 120 ml 18 ml 6 ml AEC Chromogen (20x) 1 x 8ml 1 x 4ml 1 x 2ml AEC Substrate (20x) 1 x 4ml 1 x 2ml 1 x 1ml Aqueous Mounting Medium 1 x 70ml 1 x 18ml 1 x 6ml DoubleStain Blocker 1 x 60ml 1 x 18ml 1 x 18ml Goat AP Polymer 1 x 60ml 1 x 18ml 1 x 6ml Hydrogen Peroxide (20x) 1 x 4ml 1 x 2ml 1 x 2ml Mouse HRP (AEC) Polymer 1 x 60ml 1 x 18ml 1 x 6ml Ready to Use BCIP/NBT 1 x 120ml 1 x 18ml 1 x 6ml -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-Fr |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration.
|
Notes |
---|
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab183271 has not yet been referenced specifically in any publications.