Overview

  • Product name

    DoubleStain IHC kit: G&M on human tissue (BCIP & AEC)
  • Assay type

    Polymer Multiplex
  • Product overview

    The DoubleStain IHC kit (ab183271) is designed to use with user supplied goat and mouse antibodies to detect two distinct antigens on human tissue or cell samples. This kit has been tested in paraffin tissues, however it can also be used on frozen specimens and freshly prepared monolayer cell smears.

     

    Double staining is a common method used in immunohistochemistry for the detection of two distinct antigens in a single tissue. Abcam's DoubleStain IHC Kit supplies two polymer enzyme conjugates: AP polymer anti-Goat IgG and HRP(AEC) polymer anti-Mouse IgG with two distinct substrates/chromogens, BCIP/NBT (Purple) and AEC (Red). Users will apply the two enzyme conjugates onto the specimen sequentially. If only the anti-goat antigen is present only the BCIP/NBT(Purple) will be present and if the mouse antigen is present only the AEC(Red) will be present. Abcam's DoubleStain IHC Kit is a non-biotin system avoiding endogenous biotin non-specific binding.

  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 12 ml 36 ml 120 ml
    AEC Chromogen (20x) 1 x 2ml 1 x 4ml 1 x 8ml
    AEC Substrate (20x) 1 x 1ml 1 x 2ml 1 x 4ml
    Aqueous Mounting Medium 1 x 7ml 1 x 18ml 1 x 70ml
    DoubleStain Blocker 1 x 6ml 1 x 18ml 1 x 60ml
    Goat AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Hydrogen Peroxide (20x) 1 x 1ml 1 x 2ml 1 x 4ml
    Mouse HRP (AEC) Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Ready to Use BCIP/NBT 1 x 7ml 1 x 18ml 1 x 120ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Protocols

References

ab183271 has not yet been referenced specifically in any publications.

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