Product nameDoubleStain IHC Kit: G&M on human tissue (Green/HRP & AP/Red)
Assay typePolymer Multiplex
Abcam's DoubleStain IHC kit (ab183272) is designed to use with user supplied goat and mouse antibodies to detect two distinct antigens on human tissue or cell samples. This kit has been tested in paraffin tissue, however, it can be also be used on frozen specimen and freshly prepared monolayer cell smears.
Double staining is a common method used in immunohistochemistry for the detection of two distinct antigens in a single tissue. Abcam's DoubleStain IHC kit supplies two polymer enzyme conjugates: Goat AP polymer and Mouse HRP polymer with two distinct substrates/chromogens, Emerald (Green) and Permanent Red (Red). Simplified steps offer a convenient protocol as the enzyme conjugates are sequentially applied to the specimen. A second advantage of this kit is that it allows the researcher to visualize two co-localized proteins and due to the color change when the chromogens overlap this can be semi-quantitative. For example, if the area of co-localization stains blue, the antigen indicated by Emerald is expressed at a higher concentration in the cell and if the color is purple, the antigen indicated by Permanent-Red is expressed at higher concentrations. Abcam's DoubleStain IHC kit is a non-biotin system avoiding endogenous biotin non-specific binding.
Tested applicationsSuitable for: IHC-P, IHC-Frmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 12 ml 36 ml 120 ml — DoubleStain Blocker 1 x 6ml 1 x 18ml 1 x 60ml Emerald Chromogen 1 x 7ml 1 x 18ml 1 x 70ml Goat AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Mouse HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Non-aqueous Mounting Medium 1 x 6ml 1 x 18ml Permanent Red Activator (5x) 1 x 3ml 1 x 7.2ml 1 x 14ml Permanent Red Chromogen (100x) 1 x 150µl 1 x 360µl 1 x 0.7ml Permanent Red Substrate 1 x 15ml 2 x 18ml 1 x 70ml Plastic pipette 1 unit 1 unit 1 unit
Our Abpromise guarantee covers the use of ab183272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
ab183272 has not yet been referenced specifically in any publications.