Overview

  • Product name
    DoubleStain IHC Kit: G&Rt on human/mouse tissue (BCIP & AEC)
  • Assay type
    Polymer Multiplex
  • Product overview

    The DoubleStain IHC kit (ab183281) is designed to be used with user supplied goat and rat primary antibodies to detect two distinct antigens on human or mouse tissue or cell samples. The kit has been tested on paraffin embedded human and mouse tissues, however, it can also be used on frozen specimen and freshly prepared monolayer cell smears.

    Double staining is one of the most common methods used in immunohistostaining allowing the detection of two distinct antigens in a single tissue. Abcam's DoubleStain IHC Kit (ab183281) supplies the user with two polymer enzyme conjugates: HRP polymer anti-Goat IgG and AP polymer anti-Rat IgG with two distinct substrates/chromogens: BCIP/NBT (Purple) and AEC (Red). Users sequentially apply the two enzyme conjugates onto the specimen. When two proteins are present a purple/red color will develop depending on the presence and location of the antigens the two colors should be distinct. If only the anti-goat antigen is present only Red will be visible and if the anti-Rat antigen is present Purple will be present. The kit is non-biotin system avoiding endogenous biotin non-specific binding.

  • Tested applications
    Suitable for: IHC-Fr, IHC-Pmore details

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 12 ml 36 ml 120 ml
    AEC Chromogen (20x) 1 x 2ml 1 x 4ml 1 x 6ml
    AEC Substrate (20x) 1 x 1ml 1 x 2ml 1 x 3ml
    Aqueous Mounting Medium 1 x 7ml 1 x 18ml 1 x 70ml
    DoubleStain Blocker 1 x 6ml 1 x 18ml 1 x 60ml
    Goat HRP (AEC) Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Hydrogen Peroxide (20x) 1 x 1ml 1 x 2ml 1 x 3ml
    Rat AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Rat Primer 1 x 6ml 1 x 18ml 1 x 60ml
    Ready to Use BCIP/NBT 1 x 15ml 1 x 18ml 1 x 70ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Protocols

References

ab183281 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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