Overview

  • Product name
    DoubleStain IHC Kit: G&Rt on human/mouse tissue (DAB & AP/Red)
  • Assay type
    Polymer Multiplex
  • Product overview

    Abcam’s DoubleStain IHC kit (ab183280) is designed to use with user supplied goat and rat primary antibodies to detect two distinct antigens on human or mouse tissue or cell samples. . This kit has been tested on paraffin embedded tissue, however it can also be used on frozen specimens or freshly prepared monolayer cell smears.

    Double staining is one of most common methods used in immunohistostaining allowing the detection of two distinct antigens in a single tissue. Abcam's DoubleStain IHC Kit (ab183280) supplies the user with two polymer enzyme conjugates: HRP polymer anti-Goat IgG and AP polymer anti-Rat IgG and two distinct substrates/chromogens, DAB (brown) and Permanent Red (red). Users will sequentially apply the two enzyme conjugates onto the specimen. When two proteins are present a brown/red color will develop depending on the presence and location of the antigens the two colors should be distinct. If only the anti-goat antigen is present only the DAB brown chromogen will be seen and if only the Rat antigen is present only the Permanent Red chromogen will be visible. The Kit is a non-biotin system avoiding endogenous biotin non-specific binding.

  • Tested applications
    Suitable for: IHC-Fr, IHC-Pmore details

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 12 ml 36 ml 120 ml
    Aqueous Mounting Medium 1 x 7ml 1 x 18ml 1 x 70ml
    DAB Chromogen (20x) 1 x 1.5ml 1 x 2ml 1 x 6ml
    DAB Substrate 1 x 12ml 2 x 15ml 1 x 70ml
    DoubleStain Blocker 1 x 6ml 1 x 18ml 1 x 60ml
    Goat HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Permanent Red Activator (5x) 1 x 3ml 1 x 7.2ml 1 x 14ml
    Permanent Red Chromogen (100x) 1 x 150µl 1 x 360µl 1 x 0.7ml
    Permanent Red Substrate 1 x 15ml 2 x 18ml 1 x 70ml
    Rat AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Rat Primer 1 x 6ml 1 x 18ml 1 x 60ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Protocols

References

ab183280 has not yet been referenced specifically in any publications.

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