Overview

  • Product name
    DoubleStain IHC Kit: M&M on rodent tissue (BCIP & AEC)
  • Assay type
    Polymer Multiplex
  • Product overview

    Abcam's Doublestain IHC Kit (ab183274) is designed to use with two user-supplied mouse antibodies to detect two distinct antigens on mouse and rat tissue or cell samples. Specimens can be frozen, paraffin embedded, or freshly prepared monolayer cell smears.

    Double staining is a common method used in immunohistochemistry that allows for detection of two distinct antigens in a single tissue. This kit uses an HRP or AP polymer based technology combined with a proprietary blocking buffer system that achieves ultra-sensitivity with no background or cross reactivity. Abcam's Doublestain IHC Kit (ab183274) supplies the user with a primer system to enhance the two polymer enzyme conjugates; Mouse HRP (AEC) Polymer and Mouse AP Polymer with two distinct substrates/chromogens: AEC and BCIP/NBT. BCIP/NBT reacts with Mouse AP Polymer conjugate to produce a purple color. AEC chromogen reacts with Mouse HRP (AEC) Polymer conjugate to produce a red color. Abcam's Doublestain IHC Kit (ab183274) is a non-biotin system that avoids the extra steps involved in blocking non-specific binding due to endogenous biotin.

  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 12 ml 36 ml 120 ml
    AEC Chromogen (20x) 1 x 2ml 1 x 4ml 1 x 8ml
    AEC Substrate (20x) 1 x 1ml 1 x 2ml 1 x 4ml
    Antibody Blocker (40x) 2 x 15ml 1 x 50ml 1 x 100ml
    Aqueous Mounting Medium 1 x 7ml 1 x 18ml 1 x 70ml
    Blocker A 1 x 6ml 1 x 18ml 1 x 60ml
    Blocker B 1 x 6ml 1 x 18ml 1 x 60ml
    Hydrogen Peroxide (20x) 1 x 1ml 1 x 2ml 1 x 4ml
    Mouse AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Mouse HRP (AEC) Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Mouse Primer 1 x 6ml 1 x 18ml 1 x 60ml
    Ready to Use BCIP/NBT 1 x 7ml 1 x 18ml 1 x 60ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Protocols

References

ab183274 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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