Product nameDoubleStain IHC Kit: M&M on rodent tissue (BCIP & AEC)
Assay typePolymer Multiplex
Abcam's Doublestain IHC Kit (ab183274) is designed to use with two user-supplied mouse antibodies to detect two distinct antigens on mouse and rat tissue or cell samples. Specimens can be frozen, paraffin embedded, or freshly prepared monolayer cell smears.
Double staining is a common method used in immunohistochemistry that allows for detection of two distinct antigens in a single tissue. This kit uses an HRP or AP polymer based technology combined with a proprietary blocking buffer system that achieves ultra-sensitivity with no background or cross reactivity. Abcam's Doublestain IHC Kit (ab183274) supplies the user with a primer system to enhance the two polymer enzyme conjugates; Mouse HRP (AEC) Polymer and Mouse AP Polymer with two distinct substrates/chromogens: AEC and BCIP/NBT. BCIP/NBT reacts with Mouse AP Polymer conjugate to produce a purple color. AEC chromogen reacts with Mouse HRP (AEC) Polymer conjugate to produce a red color. Abcam's Doublestain IHC Kit (ab183274) is a non-biotin system that avoids the extra steps involved in blocking non-specific binding due to endogenous biotin.
Tested applicationsSuitable for: IHC-P, IHC-Frmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 12 ml 36 ml 120 ml AEC Chromogen (20x) 1 x 2ml 1 x 4ml 1 x 8ml AEC Substrate (20x) 1 x 1ml 1 x 2ml 1 x 4ml Antibody Blocker (40x) 2 x 15ml 1 x 50ml 1 x 100ml Aqueous Mounting Medium 1 x 7ml 1 x 18ml 1 x 70ml Blocker A 1 x 6ml 1 x 18ml 1 x 60ml Blocker B 1 x 6ml 1 x 18ml 1 x 60ml Hydrogen Peroxide (20x) 1 x 1ml 1 x 2ml 1 x 4ml Mouse AP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Mouse HRP (AEC) Polymer 1 x 6ml 1 x 18ml 1 x 60ml Mouse Primer 1 x 6ml 1 x 18ml 1 x 60ml Ready to Use BCIP/NBT 1 x 7ml 1 x 18ml 1 x 60ml
Our Abpromise guarantee covers the use of ab183274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
ab183274 has not yet been referenced specifically in any publications.