Product nameDoubleStain IHC Kit: M&R on human tissue (BCIP & AEC)
Abcam’s DoubleStain IHC Kit: M&R on human tissue (BCIP & AEC) (ab210060) is designed to detect two distinct antigens on human tissue or cell samples using a simplified protocol with user-supplied mouse and rabbit antibodies. This kit has been tested in paraffin embedded tissues. However, this kit can be used to stain frozen specimen and/or freshly prepared monolayer cell smears.
Double staining is a common method used in immunohistochemistry to evaluate two distinct antigens in a single tissue. This kit contains a HRP-Polymer anti-Mouse IgG, an AP-Polymer anti-Rabbit IgG, and two distinct chromogens. The AEC chromogen (red color) is used with HRP Polymer anti-Mouse IgG and the BCIP/NBT chromogen (purple/blue color) is used with AP Polymer anti-Rabbit IgG. DoubleStain IHC Kit: M&R on human tissue (BCIP & AEC) is a non-biotin system that avoids the extra steps involved in blocking non-specific binding due to endogenous biotin.
Tested applicationsSuitable for: IHCmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 12 ml 36 ml 120 ml AEC Chromogen (20X) 1 x 2ml 1 x 4ml 1 x 12ml AEC Substrate Buffer (20X) 1 x 1ml 1 x 2ml 1 x 6ml Rabbit AP polymer 1 x 6ml 1 x 18ml 1 x 60ml BCIP/NBT Solution 1 x 12ml 2 x 18ml 1 x 120ml Mouse HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml Hydrogen Peroxide (20x) 1 x 1ml 1 x 2ml 1 x 6ml Aqueous Mounting Medium 1 x 12ml 2 x 18ml 1 x 120ml
Our Abpromise guarantee covers the use of ab210060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC||Use at an assay dependent concentration.|
ab210060 has not yet been referenced specifically in any publications.