DoubleStain IHC Kit: M&R on human tissue (BCIP & AEC) (ab210060)

Overview

  • Product name
    DoubleStain IHC Kit: M&R on human tissue (BCIP & AEC)
  • Sample type
    Tissue
  • Product overview

    Abcam’s DoubleStain IHC Kit: M&R on human tissue (BCIP & AEC) (ab210060) is designed to detect two distinct antigens on human tissue or cell samples using a simplified protocol with user-supplied mouse and rabbit antibodies. This kit has been tested in paraffin embedded tissues. However, this kit can be used to stain frozen specimen and/or freshly prepared monolayer cell smears. 


    Double staining is a common method used in immunohistochemistry to evaluate two distinct antigens in a single tissue. This kit contains a HRP-Polymer anti-Mouse IgG, an AP-Polymer anti-Rabbit IgG, and two distinct chromogens. The AEC chromogen (red color) is used with HRP Polymer anti-Mouse IgG and the BCIP/NBT chromogen (purple/blue color) is used with AP Polymer anti-Rabbit IgG. DoubleStain IHC Kit: M&R on human tissue (BCIP & AEC) is a non-biotin system that avoids the extra steps involved in blocking non-specific binding due to endogenous biotin.


     

  • Tested applications
    Suitable for: IHCmore details

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 12 ml 36 ml 120 ml
    AEC Chromogen (20X) 1 x 2ml 1 x 4ml 1 x 12ml
    AEC Substrate Buffer (20X) 1 x 1ml 1 x 2ml 1 x 6ml
    Rabbit AP polymer 1 x 6ml 1 x 18ml 1 x 60ml
    BCIP/NBT Solution 1 x 12ml 2 x 18ml 1 x 120ml
    Mouse HRP Polymer 1 x 6ml 1 x 18ml 1 x 60ml
    Hydrogen Peroxide (20x) 1 x 1ml 1 x 2ml 1 x 6ml
    Aqueous Mounting Medium 1 x 12ml 2 x 18ml 1 x 120ml

Applications

Our Abpromise guarantee covers the use of ab210060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC Use at an assay dependent concentration.

Protocols

References

ab210060 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab210060.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
For licensing inquiries, please contact partnerships@abcam.com

Sign up